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<br />The influence of migratory spawning runs of trout was avoided by exclud- <br />ing all streams which appeared to contain spawning trout from analysis. <br />The assumption that the second sample is random was addressed by Seber <br />(1973) such that if all animals have the same catchability, a random sample <br />can be approximated if each area in the population has the same probability <br />of being sampled. All electrofishing was done with the intention of shocking <br />all available trout habitat on each run. In addition, the methods for col- <br />lecting trout on the second set of electrofishing runs matched as closely as <br />possible methods on the first. The consistent sampling methods eliminated <br />any systematic differences in within and between week population estimates. <br />The assumptions of no tag loss and all tags reported could have been <br />violated and population estimates may have been biased but the bias should <br />have been consistent in within and between week estimates, i.e., both estim- <br />ates should have been similarly affected so that although biased, both <br />estimates should still have been the same. The loss of jaw tags could have <br />caused the between week estimates to be higher than within week estimates, <br />however, on the Big Horn River, tag loss over a 6 month period (May - Novem- <br />ber) was measured at only 3.4%. Also, batch marks were often used in place <br />of jaw tags. Although some regeneration of batch marks was evident, this <br />WaS easily detectable. Violation of these assumptions did not appear to <br />account for the difference between within and between week population estim- <br />ates. <br />Lagler (1968) reported that small fish were generally less vulnerable <br />to capture using electrofishing gear than were larger fish. This problem <br />will create bias in population estimates when data for all sizes of fish are <br />pooled. However, pooling creates a consistent bias between within and be- <br />tween week population estimates as long as the size of fish collected does <br />not differ between runs. On the North Platte River at Bessemer Bend, the <br />population estimates were stratified into three size groups to eliminate some <br />of the size selective bias and results showed between week estimates to be <br />larger than within week estimates. Although violation of this assumption <br />does cause biased estimates, the bias should be consistent for within and <br />between week estimates, yielding similar estimates. <br />Differences in the probability of capture of marked and unmarked trout <br />appears to be responsible for most of the difference between within and be- <br />tween week population estimates. Schreck, et al. (1976) and Whaley (1975) <br />reported on physiological changes in rainbow following electroshock and <br />concluded that more than six hours were required for a fish to return physio- <br />logically to "normal" preshock conditions. Cross and Stott (1975) working <br />with roach (Rutilus rutilus (L.)) and gudgeon (Gobio gobio (L.)) in ponds <br />showed population estimates varied depending on the length of time between <br />mark and recapture runs. They attributed differences to a learned avoidance <br />behavior of fish that were shocked, but escaped during the mark run and were <br />less catchable during the recapture run. <br />Observations made while electrofishing small Wyoming streams where both <br />mark and recapture runs were done several hours apart on the same day have <br />supported the contention that marked trout swim to the electrode much less <br />vigorously, and remain at the positive electrode for shorter periods of time <br />than unmarked trout. <br /> <br />26 <br />