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index called weighted useable area (WUA), the summation of cell areas weighted by its <br />suitability index. When plotted versus discharge WUA typically peaks at a single flow <br />that is considered the flow that maximizes habitat. PHABSIM has been criticized <br />because of assumptions implicit with micro-habitat suitability curves, assumptions of <br />positive relationships between habitat availability and fish abundance, and the tendency <br />of users to recommend flows at the peak of the WUA-discharge relation regardless of <br />natural flow occurrence. These assumptions have been obstacles for using PHABISM to <br />model impacts of reduced flows on large warm water rivers of the west slope (Rose and <br />Hahn 1989). <br />The Meso-Habitat method of this study is also an integration of hydraulic and <br />biologic analyses. We used a 2-D flow model to simulate depths and velocity. Fish <br />habitat suitability was derived from density estimates made at each site. Two- <br />dimensional (2-D) flow models have been found to have high potential for application in <br />instream flow studies (Leclerc et al., 1995; Bovee, 1996; Kondolf et al. 2000). Two- <br />dimensional models offer a significant improvement over one-dimensional (1-D) <br />modeling by increasing spatial resolution. This allows for a highly accurate <br />quantification of the physical habitat for each flow of interest. 2-D models are not <br />dependent on micro-habitat suitability curves for predicting habitat availability. Meso- <br />habitat scale precision is more efficient for sampling biological data. Criteria can be <br />established for individual species and also by guilds (sympatric species) (Parasiewicz <br />2001). A relationship between habitat availability to fish abundance assumes that fish <br />distribute themselves in the river primarily as a function of habitat. A large part of this <br />study was to develop meso-habitat criteria for two native suckers. This was <br />accomplished by examining the relationship between their abundance and meso-habitat <br />availability. The output of the meso-habitat methodology was the relationship among <br />meso-habitat availability and the range of modeled flows. <br />Species Used for the Biological Analysis <br />The primary species used in the instream flow analysis were the bluehead sucker <br />(Catostomus discobolus), flannelmouth sucker (Catostomus latipinnis) and roundtail <br />chub (Gila robusta ). These species, quite common in the Colorado River, comprised <br />about 80 percent of fish sampled between Rifle and Palisade. Anderson (1977) <br />speculated that fishery abundance was likely at carrying capacity set by the physical <br />habitat. Samples made by the Recovery Program (ISMP) in the Colorado River <br />downstream of Grand Junction to Loma found native fish were about 90% of the catch <br />(Ehnbald 2003). Osmundson (1999) reported high catch rates of native suckers in the <br />Colorado River, with the highest catch rate in the 15-Mile Reach. The thriving <br />populations of these three native species suggest that habitat, base flow alternations or <br />non-native introductions have not negatively impacted their life history requirements in <br />the river. In fact, studies suggest the Colorado River is an excellent location to study <br />dynamics between these three native fish and physical habitat. <br />13 <br />