7606

• UCREFRP Catalog Number: 7606
• Author: Negus, M. T., D. A. Belford and S. E. Colvin.
• Title: Long-term Retention of Fluorescent Pigment Marking of Chinook Salmon.
• USFW Year: 1990.
• USFW - Doc Type: St. Paul, MN.
• Copyright Material: NO

of the same stock. All pelvic-clipped spray-marked and
control fish were stocked into the French River, a
tributary of Lake Superior.
Marked chinook salmon were captured in the French
River trap, located approximately 100 m upstream from Lake
Superior, during the 1986 through 1989 spawning migrations.
Fin-clipped fish were weighed, measured, and sexed, and
scale samples were taken. Fish with right pelvic clips
were examined under ultraviolet light; the presence and
locations of fluorescent dye granules were noted.
Survival of spray-marked versus control fish was
compared using a Heterogeneity Chi-square test (Snedecor
and Cochran 1973). Growth of spray-marked and control fish
was compared by back-calculating lengths in each year of
life (Missouri Department of Conservation 1989). A body-
scale constant of 38 mm was used in back-calculations. A
two-way analysis of variance (ANOVA) of total body length
in each year of life was done using mark (spray-marked
versus control) and sex as the independent variables
(Snedecor and Cochran 1973). The Kolmogorov-
Smirnov/Lilliefors test was used to check for normality
within each cell of the ANOVAS (Lilliefors 1967). Levene's
test was used to check for homogeneity of variance within
each cell (Levene 1960). Hypotheses were tested at the
0.05 level. Length-weight relationships at capture of
spray-marked versus control fish were compared using
analysis of covariance (ANCOVA) on log transformed data.

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