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<br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br /> <br />CHAPTER l--INVERTEBRA TES <br /> <br />By Lewis Boobar (NPS) <br /> <br />METHODS <br /> <br />A total of 16 Surber (30 X 30 cm sample area, 500 um net) samples (Merritt and Cummins 1996) <br />were collected from riffles along the lower Escalante River. Two of the 16 samples were <br />collected from each of four riffles (sites), four from the upper and four from the lower reaches <br />(Map 1). Additionally, 30 minutes were spent at each site collecting macroinvertebrates from <br />debris and snags. The samples and associated water were mixed with 95% ethyl alcohol to <br />obtain a concentration (ca. 50% ethyl alcohol) high enough to temporarily preserve the animals. <br />Later, samples were composited by type and river-section. The resulting two composite Surber <br />samples and two composite qualitative samples (one of each type from each river-section) were <br />processed by the Bureau of Land Management and U.S. Forest Service National Aquatic <br />Monitoring Center at Utah State University, Logan, UT as described by Vinson and Hawkins <br />(1996). Initially, the entire sample was sorted for large and less-numerous organisms. If the <br />sample contained more than 250 organisms, it was sub-sampled. They took additional <br />sub-samples until at least 250 organisms are found. The identified portion of the sample was <br />placed in 70% ethyl alcohol, cataloged, and archived. The data was entered into a common <br />computer database at the National Aquatic Monitoring Center. This database currently contains <br />aquatic macroinvertebrate data and corresponding geographic information for more than 4000 <br />samples. <br /> <br />RESULTS <br /> <br />A total of 475 macroinvertebrates representing 19 taxa were collected, 116 from the upper reach <br />and 359 from the lower reach (Table 1.1, Appendix A). The dominant families were <br />Chironomidae, Hydrosychidae, Simuliidae and Tricorythidae (Table 1.2). Diversity was fairly <br />constant throughout the study area as was the Modified HilsenhoffBiotic Index <br />(MHBI)(Hilsenhoff 1987, 1988; Bode et al. 1991), which summarizes the overall pollution <br />tolerances of the taxa collected, and the USGS Community tolerant quotient (Wingett and <br />Mangum 1979). The taxa richness (Table 1.3) and abundance (Table 1.4) of functional feeding <br />groups showed collector gatherers and collector filters to be slightly greater in the lower reach, <br />and scrapers to be slightly greater in the upper reach. <br /> <br />4 <br />