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<br />I <br />I <br />I <br />I, <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />, <br />I <br />I <br />I <br />'I <br />I <br />I <br />I <br /> <br />Light Traps. Quatrefoil style light traps were used to collect larval fishes (Mueller et al. 1993). <br />Entrance widths were originally 10 mm to facilitate capturing larger native juveniles, however, <br />potential predators such as adult red shiners and small sunfish also entered. Less than 30 larvae <br />were captured during the 1999 season. The entrances were reduced to 5 mm to exclude predators <br />during the 2000 field season. The traps had self-draining plankton nets to concentrate the <br />samples and three 300-mA light bulbs (total 0.9 A) with two "D" cell batteries that provided <br />light for 1 0 hours. Lights were placed in a clear 3-cm plexiglass cylinder. Two traps were set <br />before dark along shore or in the backs of coves and recovered early in the morning. CPUE was <br />expressed in numbers of larvae per night set (larvae/set). <br /> <br />Seining. Shoreline and backwater habitats were seined at the inflow sites using a nylon 4-mm <br />meshed 1.5-m by 12-m seine. The seine was pulled going downstream (if current was present) <br />and the area sampled (m2) was estimated. CPUE was expressed as the number of fish seined per <br />10 square meters (fish/l 0 m2). <br /> <br />Acoustics. Pelagic fish densities were measured using a scientific echo sounder (BioSonics <br />Model DT-5000). The system operated at 420 KHz and multiplexed between single and dual- <br />beam transducers that were mounted on an aluminum platform on the side the boat. Permanent <br />acoustical transect stations were established at RM 36, 38,40,42,44,46,48,50, and 52 on the <br />San Juan arm. Addition transect data were collected at Spencer's Camp, Nokai Canyon, Castle <br />Creek Canyon, and Mike's Canyon. Transects started at shore and proceeded to the thalweg. A <br />minimum effort of 1,000 pings (approximately 5 minutes of mobile scanning) was collected at <br />each site. Analyzed data provided size distribution and standing crop information (fish/m3 and <br />kg/m3). <br /> <br />Sampling Effort <br /> <br />During the 4 sampling days per field trip, a crew of five to eight biologists set a minimum of two <br />minnow traps, two small-mesh (6 mm) hoop nets, two large-mesh (2.5 cm) hoop nets, two large <br />(3.7-cm) trammels, and two small-mesh (12 mm) trammels per night at the San Juan inflow and <br />Spencer's Camp sites. Similar effort occurred at the Colorado River inflow, however, the <br />number and types of nets used depended upon netting conditions and set opportunities. We <br />seined (10 pulls) once each inflow trip and set light traps (May-July). All areas were <br />electrofished using standardized methods developed by the UDWR. Each site was sampled one <br />night and the effort consisted of three, IS-minute shocking periods. Occasionally sampling was <br />interrupted due to bad weather, equipment breakdown, or when we experienced high catch rates <br />(> 1 00 fish/set) for spawning carp and threadfin shad. All fish were released unharmed except <br />for a total of 40 fish used for a heavy metal analysis (Jerry Miller, BaR). Field trips typically <br />consisted of 4 sampling days. <br /> <br />Acoustical surveys were conducted before (May) and after the spawning peak (June-August). <br />All surveys were conducted during daylight hours and took approximately 4 to 6 hours to <br /> <br />7 <br />