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<br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br /> <br />the principal identifiable food items. Digestive tracts of 22 (5%) red shiner adults contained fish <br />larvae; 9 from Shafer Canyon (4%), 2 from Lathrop Canyon (8%), 5 from Millard Canyon (18%), <br />and 6 from the Bonita Bend area (3%). All fish larvae consumed were identified as cypriniforms <br />and most were catostomids (species undetermined because larvae were too digested for <br />accurate identification). <br />The only other study in the Colorado River basin to document pr~ation by red shiner on <br />fish larvae was that conducted by Ruppert et al. (1993). They exami~~d t~ diets of 184 red <br />shiner (36-79 mm TL), 47 sand shiner Notropis stramineus (30-65), 4~thead minnow <br />Pimephales promelas (32-60), 176 redside shiner Richardsonius balteatus (36-77), and 17 <br />channel catfish Ictalurus punctatus (51-144) collected during 30 June-25 July 1991 from <br />ephemeral shoreline embayments near the confluence of the Yampa and Green rivers, <br />Colorado. Fish larvae were found in 15% of the red shiner but not in any other species. Of the <br />58 fish larvae consumed, all were cypriniforms (mostly catostomids); seven were identified as <br />bluehead sucker. Insects, including adult or immature caddisflies, mayflies, beetles, and water <br />striders, were the principal food items in digestive tracts of all fishes except fathead minnow, <br />which contained mostly algae and organic debris. Ruppert et al. (1993) concluded that <br />because red shiner are very abundant in nursery habitats used by young native fishes, they <br />may be an important predator on native fishes in the Colorado River system, especially in <br />habitats with low invertebrate forage during spring and early summer. <br /> <br />Evaluation of Adult Red Shiner Exclosures: 1995 (Objective 2) <br /> <br />Sampling outside and inside each exclosure (one inside Millard Canyon and one in a <br />flooded wash at Bonita Bend) demonstrated that adult red shiner were successfully excluded <br />from the exclosed areas, but larval fish (including suckers) easily passed through the block <br />netting. The integrity of the exclosure inside Millard Canyon was successfully maintained until <br />late June when it was breached by rising water levels, whereas, the integrity of the exclosure at <br />Bonita Bend was maintained throughout the sampling season. Results suggest that use of <br />exclosures to block access of nonnative fishes into important native-fish nursery habitats could <br />be adapted for broader-scale use in the Recovery Program. In a recent review of options for <br />selective control of nonnative fishes in the upper Colorado River basin, Lentsch et al. (1996) <br />concluded that mechanical control of many of the smaller non natives in critical nursery habitats <br />for endangered fishes might be achieved by blocking their access into portions of these <br />habitats. <br /> <br />Marking Otoliths in Larval Suckers: 1995 (Objective 3) <br /> <br />Otoliths from all flannelmouth sucker larvae treated with TC had lucid or bright <br />fluorescent marks, suggesting that marking of otoliths with fluorescent chemicals could be <br />applied in field mark-capture studies on razorback sucker larvae to facilitate investigation of <br />their early biology and recruitment. Number of distinct growth increments in otoliths <br />cqrresponded directly to the number of days between marking and subsampling, demonstrating <br />that otoliths of larval f1annelmouth sucker deposit daily growth increments. If flannelmouth <br />sucker larvae are appropriate surrogates for razorback sucker in determining patterns of otolith <br />growth, then daily deposition of growth increments could be assumed for wild razorback sucker <br />larvae. Results of a recent laboratory study conducted by the LFL on growth of otoliths in <br />captive larval razorback sucker confirmed daily deposition of growth increments. With these <br />field and laboratory validations of otolith growth, wild-caught razorback sucker larvae can be <br /> <br />27 <br />