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Final Report 2-3 September 2000 reliable data for refining the flow recommendations in the 1992 Biological Opinion, and (3) investigate potential effects of increasing the temperature of water released from Flaming Gorge Dam. The four major objectives of the investigation were to: 1. Track reproduction of the endangered fishes in the Green and lower Yampa Rivers and determine relationships among seasonal flows, water temperatures, and reproductive success; 2. Evaluate recruitment of the endangered fishes from age 0 to subsequent life-history stages and determine relationships among seasonal flows, water temperatures, and survival of young fish; 3. Monitor the relative abundance and population structure of the endangered fishes in order to acquire information on interactions among fish species and how flows may differentially affect populations; and 4. Determine how releases from Flaming Gorge Dam and flows from tributaries affect the formation and maintenance of important habitats for the endangered fishes throughout the Green River. When designing the Flaming Gorge Flow Recommendations Investigation, the following considerations were deemed important by the research team: . The research program should address all endangered fishes to the extent possible. Nonnative and other native fishes should also be considered, especially in terms of their interactions with endangered fishes. Endangered fishes included humpback chub, Colorado pikeminnow, and razorback sucker. . Long-term, standardized studies extending for the length of the research period would be needed to provide a database for assessment of the biological and physical responses of the Green River ecosystem to implemented flow recommendations. . The framework of the long-term effort should be sufficiently simple and flexible to allow for continuity over time and collection of accurate quantitative data covering important life-history stages. Consideration was given to the ability to link new data with existing data and information generated from the Interagency Standardized Monitoring Program (ISMP) and other studies. Research within the Flaming Gorge Flow Recommendations Investigation should be coordinated with other studies or programs in the Recovery Program such as ISMP, channel monitoring, and hydrology support and with other research activities in the Green River funded outside of the Recovery Program.