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<br />film, and then covered with immersion oil for aging. Daily increments in each otolith were <br />counted three times on separate occasions at 1000X magnification, and capture date, fish length, <br />and otolith diameter were unknown, Counts were averaged for each larva to arrive at an <br />estimated age in days posthatching. Horn (1996) determined that the first increment in otoliths <br />of larval razorback suckers forms at hatching, and increment deposition proceeds at rate of <br />approximately one increment per day. <br />Spawning dates for razorback suckers in the middle or lower Green River each year <br />during 1993-1996 were estimated by subtracting estimated incubation times of embryos from <br />hatching dates of larvae. The hatching (date for an individual larva was established by subtracting <br />its posthatching age from its date of capture. Temperature-dependent incubation times of <br />embryos at ambient Green River temperatures on and closely preceding hatching dates were <br />estimated using data presented by Marsh (1985) and Bozek et al. (1990) for captive embryos. <br />Mainstem water temperature and discharge parameters selected for association with <br />spawning dates in each river section (middle or lower) and year included (1) mean (range) <br />discharge and temperature during the spawning period; (2) degree days, which was the sum of <br />recorded instantaneous daily water temperatures between 1 January and the earliest date of <br />spawning; (3) days ~ lOoC or ~ l40C, which were the number of days between 1 January and the <br />earliest date of spawning that recorded instantaneous daily water temperatures equaled or <br />. exceeded each respective threshold; and (4) days before peak discharge, which was the number <br />of days between the earliest date of spawning and the highest recorded mean daily river <br />discharge. Missing temperature data were estimated by linear interpolation, For this analysis, we <br />assumed that razorback sucker larvae caught in either the middle or lower Green River had been <br />produced locally. <br />Daily gain in total length (growth) of an individual aged razorback sucker larva between <br />hatching and date of capture was estimated by subtracting an approximate mean TL at hatching <br />of8.0 mm (Minckley and Gustafson 1982; Marsh 1985; Snyder and Muth 1990) from its total <br />length at capture and dividing by its age. Mean daily growth was calculated for larvae caught in <br />the middle or lower Green River in each year. <br /> <br />10 <br /> <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />IeI <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />