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<br />this pattern is for razorback sucker in the Colorado River below Palisade. For razorback <br />sucker and bonytail, the two fish sizes represent the estimated size of fish after the first and <br />second growing seasons. Based on monitoring results after replication of each combination <br />in each river, production may be modified in the third year to focus on the apparent optimal <br />fish size and season to stock in each river. This approach is consistent with draft <br />recommendations made by Burdick and Bonar (1997) for stocking razorback sucker. For <br />razorback sucker and bonytail, PIT tagging is not considered feasible due to cost, or <br />necessary due to the absence of riverine populations in Colorado. A less-expensive batch <br />mark for distinguishing between cohorts stocked each year would be useful for tracking year- <br />class composition and sUIVival of stocked fish in the adult population over time. For <br />Colorado pike minnow , the two sizes represent the first and subsequent sizes at which the fish <br />may be PIT tagged prior to stocking. Due to the presence of a wild population of Colorado <br />pikeminnow in the adjacent downstream reach, it is recommended that PIT tagging be used <br />on all Colorado pikeminnow stocked. The cost for this PIT tagging will range from $3,000 <br />to $9,000 per year based on 800-2,400 fish and $3.75/tag. Monitoring would require the <br />implementation of population estimate protocols (see Osmundson and Burnham 1996) for <br />adult fish in each river reach. This approach is considered preferable to ISMP (Interagency <br />Standardized Monitoring Program) adult catch/effort index until a relationship between the <br />two parameters can be established. It is also possible for mark-recapture population <br />estimates to be derived from PIT tag data collected using ISMP sampling protocols conducted <br />on any of the target reaches. This would add at least four additional monitoring reaches to <br />ISMP. <br /> <br />The target objective of stocking for each of the three species is to meet the <br />abundance/biomass based criterion with the presence of three adult age groups sUIViving in <br />each river reach. For razorback sucker, these age groups are 5-7; for bonytail, age groups <br />4-6; and for Colorado pikeminnow, age groups 6-8. The cumulative sUIVivallabundance/ <br />biomass of these age groups is desired to achieve the population objective in the river. <br />Using monitoring to evaluate sUIVivaI and abundance post-stocking, subsequent numbers of <br />fish stocked may have to be increased or decreased. The presence of an adult, multi-year- <br />class population in the target river reaches is desired to further detennine spawning and <br />reproductive success, and the effects of limiting environmental factors that may be <br />influential. The persistence of a potentially long-lived, stocked adult population would thus <br />contribute to recovery of subsequent self-sustaining wild populations of these species in these <br />river reaches. <br /> <br />Stocking of humpback chub in Colorado is not anticipated at this time. This species <br />is characteristically associated with canyon-bound river habitat, which dictates where stocking <br />would occur. Within the two canyon reaches known to support humpback chub in Colorado, <br />Yampa Canyon on the Yampa River and Black Rocks on the Colorado River, research has <br />failed to discover a significant threat of extirpation to either population under current <br />environmental conditions. This includes the presence of an abundant and potentially <br />predatory channel catfish population in both reaches. The Black Rocks population has <br />demonstrated a relative stability since monitoring began in the early 1980s (see . Miller et aI. <br /> <br />5 <br />