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<br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br /> <br />70 <br />owing to an incubation temperature of 180C vs. 230C for the other two experiments. At <br /> <br />fIrst feeding, all larvae were the same size. <br /> <br />As with other experiments, Ad libitum treatment larvae were by d 10 larger, <br />heavier, and contained a higher proportion of lipids than their counterparts (Table 12, <br />Figure 15 a). By d 29 Ad libitum larvae had increased to a mean size of21.2:t 0.37 nun. <br />Suboptimal and Starved larvae did not begin to differ significantly in size until 22 d. The <br />former were 14.5:t 0.2 nun by end of the experiment. Starved larvae showed an initial <br />increase in growth associated with use of yolk reserves, after which all change in length <br />ceased. Starved larvae did not survive to conclusion of the experiment (29 d). <br />Weight of Ad libitum larvae increased in a log-linear fashion (Figure 15 b). <br />Again, differences between Ad libitum larvae and the other two were apparent from 10 d <br />on. Starved and Suboptimal larvae did not differ significantly in weight until d 22 (Table <br />13). By d 29, Ad-libitum larvae averaged 6.5 mg, Suboptimal larvae, 0.67 mg (a 50% <br />increase in weight), and Starved larvae an average 0.243 mg at d 22 when the last <br />individuals died. <br /> <br /> <br />Lipids expressed a percentage body weight were difficult to interpret. Suboptimal <br /> <br /> <br />and Starved larvae never differed significantly over the course of the experiment (Table <br /> <br /> <br />14, Figure 15 c). Ad libitum larvae always had a significantly higher proportion of body <br /> <br /> <br />lipids than their counterparts except for d 22, when no treatments differed significantly <br /> <br /> <br />from one another. The dip in lipid levels on d 22 for Ad libitum larvae, was not reflected <br /> <br /> <br />in any other parameters, and was traced through analysis of feeding logs to laxity on the <br /> <br /> <br />part of assistants feeding the larvae; on at least one occasion the wrong tanks were fed. <br />