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<br />that humpback chub might be attempting to spawn in the Little Snake River. <br />Humpback chub were captured and telemetered in the Little Snake River between <br />May and July during their spawning period. Two humpback chub were captured <br />with secondary sexual characteristics such as spawning coloration and breeding <br />tubercles. Water temperatures in the Little Snake River were adequate for <br />spawning when humpback chub were present. Long-range movements of 32 and <br />39 km by two telemetered humpback chub in the Little Snake River were similar to <br />distances moved by spawning humpback chub in the Grand Canyon. Despite <br />considerable fidelity to local population centers, humpback chub are known to <br />move relatively long distances between populations groups or for spawning. <br />Although there is circumstantial evidence that support potential spawning of <br />humpback chub in the Little Snake River, currently there is no proof. No <br />humpback chub have been collected in ripe, spawning condition in the Little Snake <br />River and all juveniles collected there have been identified as congeneric roundtail <br />chub. In addition, confirmation through larval collections was not possible <br />because there were not adequate morphometric characteristics to distinguish small <br />larvae of Gila species. Differentiation of small Gila species larvae may require <br />genetic evaluation. <br /> <br />Our recommendations for the Instream Flow Work Plan focus on research <br />necessary to develop scientifically defensible flow recommendations. We <br />recommend: <br /> <br />1. Develop interim flow recommendations based on present knowledge. <br />2. Estimate Little Snake River flows and sediment loads necessary for creation <br />and maintenance of Colorado pikeminnow and razorback sucker nursery <br />habitats in the Green River. <br /> <br />viii <br />