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PROJECT INTRODUCTION/BACKGROUND <br />(In Preparation) <br />REPORT ON RESULTS OF ALLOZYME INVESTIGATIONS <br />Scope/Methods <br />IN PREPARATION. Will review sampling areas and methods for <br />project. Methods for morphological studies will be given <br />separately in each appropriate section of Douglas' appended <br />report. Methods of allozyme studies will appear here. <br />Basically, it's the standard stuff for allozyme studies that all <br />the Panel Members will be familiar with. Of pertinence now, as <br />for scoring methodology, gel bands were scored directly with <br />increasing anodal mobility (i.e., slowest band is 11111, next <br />fastest 112" & so on), EXCEPT AK-1 which was inadvertantly scored <br />in opposite fashion when rescored by Morizot and was entered in <br />thusly in data base. This makes no difference in terms of <br />analyses because scoring was consistent within each locus; Just <br />when referring to relative mobilities of alleles in discussions. <br />y/!ea ..-.•s ?Xw...?...s'./ In 7w?/? a?, ??j5'T".. ? .rr•?t.../ •q A..?/Tp/ <br />t <br />'V16-74- <br />A <br />Results <br />Eventhough chubs of the genus Gila from the Colorado and <br />adjacent basins to the south and east exhibit a considerable <br />range of phenotypic variation with regard to body shape and other <br />morphological characters, including the former occurrence of <br />three sympatric forms in the Colorado, with the respect to <br />allozymes examined, geography far transcends morphology as a' <br />correlate of genetic variation. It therefore seems prudent to <br />organize the following discussion in geographic context. The <br />overriding pattern of variation at most loci examined is one of <br />relative simplicity to the south in Mexican drainages with <br />increasing complexity northward culminating in a high degree of <br />polymorphism in the upper Colorado Basin. The following sections <br />are thus geographically arranged to reflect that trend from the <br />simple to the complex. <br />Prior to the geographic discussion of results, it should be <br />noted that 12 of the 24 loci examined throughout all samples were <br />invariate or essentially so. Eight, GAPD-1, GAPD-2, GLYDH, GR-1, <br />GR-2, MDH-1, SOD, and TPI-1 were invariate. The four remaining <br />displayed exceedingly rare alleles in one or few samples or the <br />rare variants may be attributable to electrophoretic artifacts.