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7/14/2009 5:02:34 PM
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5/22/2009 4:34:17 PM
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UCREFRP
UCREFRP Catalog Number
9327
Author
Starnes, W. C.
USFW Year
1995.
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and Santa Cruz systems. These groupings generally remained <br />intact in subsequent analyses which deleted some other <br />populations (Figs. 16,17) so long as the component samples were <br />themselves retained in the analysis. Thus, overall, while these <br />lower basin samples collectively are genetically relatively <br />similar (all grouping at the 0.95 level or above), they group <br />mosaically with only few subpatterns reflecting geography or <br />morphological groupings. <br />It is notable that some samples from the same streams <br />separated by a few kilometers failed to cluster. The two samples <br />from the upper Verde River (at Perkinsville [VDP] and Tapco <br />[VDS+VBT]), though separated by perhaps fewer than 20 stream <br />kilometers (Fig. 1), clustered in widely divergent groups (Fig. <br />15), while the samples from upper and lower Eagle Creek in the <br />upper Gila Basin were divergently placed but at a somewhat higher <br />level of similarity. Possible reasons for this and other <br />groupings will be further discussed below under Allelic Variation <br />and Taxonomic Implications. <br />DeMarais' (1992) phenetic analyses incorporated samples from <br />fewer and, in some cases, different Gila populations of the Gila <br />and Bill Williams basins compared to those reported herein. <br />Referring to populations examined by both studies., dendrograms <br />presented in his report are at variance with Figure 15 and others <br />herein at several junctures, though some (e.g., Cherry - Tonto <br />crs.; Bonito Cr. - Redfield Canyon) do cluster relatively <br />similarly. Comparison of allelic variation at loci examined in <br />both studies reveals that findings were relatively congruent with <br />minor exceptions as noted below. DeMarais examined five loci not <br />examined herein and at least one (AH-A) may have provided <br />sufficient variation to effect allignment of samples; conversely, <br />many more loci were screened herein than by DeMarais and, of the <br />24 that ultimately entered the BIOSYS analysis, at least 15 <br />differed from his, including several variable ones. The lack of <br />agreement between the respective phenetic analyses therefore may <br />be a function of both the partial differences in the suites of <br />variation analyzed and the overall mix of populations <br />represented. While offering different allignments, DeMarais' <br />analyses likewise show an overall lack of pattern with respect to <br />geography or supposed morphological groupings with some <br />exceptions (e.g., clustering of morphologically similar samples <br />from Spring, Redfield, and Bonita crs.; geographically proximate <br />Blue & San Carlos rivers, etc.). <br />Allelic Variation <br />while some loci, which were polymorphic in samples from <br />other regions studied, were monomorphic or nearly so among <br />samples from the Gila Basin (e.g., AK-1, bGAL, IDH-2, Figs. <br />2,3,8), others showed considerable variation in allele <br />frequencies. EST-2, GPI-1, MPI-2, and PGM are particularly <br />11
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