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spp. was established because knowledge of variation of <br />numerous loci (45) in this species was gained in the study. <br />1989. Moretti, M., S. Cranney and B. Roberts. Distribution and <br />abundance of Gila spp. in Desolation and Gray canyons of the <br />Green River during 1985 and 1986. Utah Div. Wildlf. Res. <br />Rpt., Salt Lake City. 29 p. <br />Reports a study in which numerous Gila spp. were collected <br />in the Green River in Desolation and Gray canyons with <br />morphometric and meristic data taken and photographs for <br />later analysis by digitizer and computer. Fin tissue and <br />dead specimens were frozen for eletrophoretic analysis. <br />Morphometric and eletrophoretic analyses of this material <br />were performed by M.J. Rosenfeld (see below). In this <br />population, based on chromosomal analysis, "reproductive <br />isolation" was indicated between G. cypha and G. robusta; <br />the degree of isolation between G. cypha and G. elegans (if <br />present?) could not be determined. However this same <br />conclusion cannot be inferred from Rosenfeld and Wilkinson <br />(1989)(next) who stated that these taxa were <br />electrophoretically indistinguishable. Morphological <br />analysis indicated considerable variation among localities <br />within the canyon area, ranging from pure humpbacks to <br />"humpback intergrades" similar to some specimens encountered <br />from Black Rocks on the Colorado River. However, it was <br />felt that hybridization was rare or non-existent (but see <br />conflicting opinions below). <br />1989. Rosenfeld, M.J., and J.A. Wilkinson. Biochemical genetics <br />of the Colorado River Gila complex (Pisces: Cyprinidae). <br />Southwest. Nat. 34:232-244. <br />Presents results of a study performed on Gila specimens from <br />` scattered localities in the Colorado basin, including <br />presumed cypha from Colorado and Green river canyon areas <br />and the Little Colorado River, robusta from the Colorado <br />River and the San Raphael system (Green R. trib.), elegans <br />obtained from the hatchery stock derived from Lake Mohave <br />population, G. robusta seminuda from the Virgin River, and <br />known hybrids from hatchery stock. Fixed differences at <br />loci were not found but allelic variation was present, <br />though not necessarily in concordence with morphotypes. <br />Intra- and interspecific allelic variation was present. G. <br />cypha and G. robusta from the upper basin are <br />indistinguishable while differences exist between upper <br />basin and Little Colorado humpback samples. While <br />heterozygosity may have remained high in elegans (e.g., <br />Minckley et al, 1989, above)(but no historic estimate of <br />this is available?), this attribute may have declined in <br />recent years in cypha and extensive hybridization with <br />robusta is inferred in upper basin populations. This is in <br />contrast to statements made in Moretti et al. above based on <br />21