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A single specimen of the virgin spinedace, Lepidomeda mollispinis, was collected from the mouth of the Paria River on July 19, 1972. A single specimen of the golden shiner, Notemi- gonus crysoleucas, was collected from Kanab Creek (Mile 143.5) on June 9, 1975. The red shiner, Notropis lutrensis, was collec- ted from five different sites. On August 16, 1971 five specimens were collected at River Mile 194.5 and one specimen from River Mile 212.5. Subse- quently no more specimens were collected above the head of the Mead Reservoir. The red shiner was collected at Spencer Creek (Mile 246) on August 5, 1975 and June 8, 1976. It was collected at Scor- pion Island (Mile 279.5) and Pierce Ferry Landing (Mile 280) on August 5 and August 6, 1975 respec- tively. The fathead minnow, Pimephales promelas, another introduced bait fish apparently can tolerate the coldwater conditions better than the red shiner which is a native of the lower Mississippi Valley. The fathead minnow was collected from 16 differ- ent sites, the uppermost locality was River Mile 44 and the lowermost was Spencer Creek (River Mile 246). It was collected five different times at the mouth of the Little Colorado, four times at Royal Arch Creek and four times at Deer Creek. The channel catfish, Ictalurus punctatus, was collected from six different sites: Little Colorado River, Cardenas Creek area (Mile 71), Shinumo Creek, Kanab Creek, River Mile 194.5, and Spencer Creek. It was collected at the Little Colorado River mouth twice, four times from mouth of Kanab Creek and once from each of the other sites. The Rio Grande killifish, Fundulus zebrinus, seems to be well established at a number of tribu- taries. This introduced killifish was collected six different times at the mouth of the Little Colorado River, three times at the Cardenas Creek and both times that we collected at Spencer Creek. The Rio Grande killifish is abundant at Spencer Creek and during the last trip on June 8, 1976, many spawning groups were observed. The Rio Grande killifish was collected from ten different sites, the uppermost at the Little Colorado and the lowermost at Spencer Creek. Miller and Lowe (1964) reported the Rio Grande killifish from the middle part of the Little Colorado River basin where it was first noticed in 1938. Minckley (1973) repeated the above record and had no addi- tional locality records although fish samples were taken from various sites along Little Colorado River and main Colorado River. When we examine our records in a chronological fashion we would have to say that we have witnessed a dramatic dis- persion in a downstream direction from the mouth of the Little Colorado down to the head end of the Mead Reservoir. The mosquitofish, Gambusia affinis, was collec- ted at two sites: Emory Falls (Mile 274.3) and Pierce Ferry Landing (Mile 280). A dead striped bass, Morone saxatilis, was ob- served floating in the Mead Reservoir at approxi- mately River Mile 250. It was just under 700 rom in total length. The green sunfish, Lepomis cyanellus, bluegill, Lepomis macrochirus, and the largemouth bass, Micropterus salmoides, were collected at Spencer Creek. In addition, the largemouth bass was col- lected at Emory Falls and Scorpion Island. '\ In conclusion we would like to reiterate and emphasize a message that Wallis gave in his paper of twenty-five years ago. He said, "The fauna is in urgent need of further research, because many forces are fast exterminating it". He cited ref- erences that stated similar messages before him. We believe that the humpback chub is in a pre- carious state, and we cannot assume that it will maintain a population in the Grand Canyon. Any form of harassment such as angling within the present known distribution should not be permitted. The study of the fish fauna should be estab- lished as a continuous long-range program. We acknowledge the following individuals for their aid in collecting specimens: Clyde Jones, James Deacon, James Williams, Dawn Remington, Lyn Branch, Cindy Deacon, Linda Loetterle, Deborah Robertson, Margaret Mathews, Pat Moss, Leah Straub and Alice Stevenson. All photographs were done by Jeanne Suttkus. LITERATURE CITED DILL, W.A. 1944. The fishery of the lower Colo rado River. California Fish and Game, 30: 309-201. HOLDEN, P.B. and C.B. STALNAKER. 1970. Syste- matic studies of the cyprinid genus Gila, in the upper Colorado River basin. Copeia, 1970(3): 409-420. HUBBS, C.L. and R.R. MILLER. 1953. Hybridization in nature between the fish genera Catostomus and Xyrauchen. Pap. Michigan Acad. Sci. Arts, Lett., 38: 207-2 33. MILLER, ROBERT R. 1946a. The need for ichthyo- logical surveys of the major rivers of western North America. Science, 104(2710) :517-519. MILLER, ROBERT R. 1946b. Gila cypha, a remarka- ble new species of cyprinid fish from the Colo- rado River in Grand Canyon, Arizona. Jour. Wash. Acad. ScL, 36(12) :409-415. MILLER, ROBERT R. and C.H. LOWE. 1964. Part 2. An annotated checklist of the fishes of Arizona. In: The vertebrates of Arizona, ed. by E.H. Lowe. Univ. Arizona Press, Tucson, Arizona, pp. 133-15l. MINCKLEY, W.L. 1973. Fishes of Arizona. Arizona Game and Fish Department. Phoenix, 292 pp. MINCKLEY, W.L. and J.E. DEACON. 1968. Southwest- ern fishes and the enigma of "endangered spe- cies". Science, 159:1424-1432. WALLIS, O.L. 1951. The status of the fish fauna of the Lake Mead national recreational area, Arizona-Nevada. Trans. Amer. Fish. Soc. 80(1): 84-92. 604