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<br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br /> <br />23 <br /> <br />(however, all seven of the deaths among these fish were <br />attributable to being suctioned onto the slotted siphon <br />tubes and not to depletion of fat reserves). At day 210, <br />only 6 of the original 90 starved medium fish were still <br />alive (19 were removed for lipid analysis on day 70 and 12 <br />on day 140). As was the case with small fish, fed medium <br />fish showed significantly higher survival than their unfed <br />counterparts. At day 210, only 2 of the original 125 <br />pellet-fed medium fish had died (27 were removed for lipid <br />analysis on day 70 and 12 on day 140). The medium fish that <br />were fed brine shrimp had 31 survivors on day 210 from the <br />original 32 fish. All large fish (mean length = 44 mm), <br />both pellet-fed and starved, were alive after 210 days <br />(notwithstanding a small number of large fish that died in <br />October-November due to the effects of ichthyobodiasis <br />(costiasis)). Large brine-shrimp-fed fish experienced high <br />mortality due to an Ichthyobodo infestation during December. <br />The surviving nine fish were sacrificed to prevent the <br />spread of the disease to the other brine-shrimp-fed fish in <br />the other aquaria. <br />Body water content is inversely related to lipid <br />content (Love 1970; Niimi 1972; Toneys 1977). Fish with <br />high fat contents tend to have lower fractions of body water <br />because only small amounts of water are required in the <br />storage of fat (Odum et al. 1964). I found that, as lipid <br />content decreased in age-O Colorado squawfish, percent body <br />water increased linearly (Figure 4). <br />