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<br />;elenium- <br />lent; N = <br /> <br />2 <br />~ <br /> <br />;) <br />9 <br />-8) <br /> <br />een seen <br />i tral ne- <br />ltion in <br />mayfly <br />1ium in <br />ytes and <br />~r archi- <br />:te infil- <br />. (l984) <br /> <br />striped <br />ue along <br />e ducts. <br />lal, with <br /> <br />-ass dis- <br />tructure <br />;den red <br />le prox- <br />re swol- <br />th casts <br />JUS ma- <br /> <br />lakes or <br /> <br />S4) <br />S4) <br />S4) <br /> <br />"; <br />., <br /> <br />3: <br /> <br />s. <br />.)'t;- <br />., <br />1 <br />;. <br />., <br /> <br />DIETARY TOXICITY AND STRIPED BASS <br /> <br />405 <br /> <br /> <br />--;~...--_. ,.,.-.' ~ . - ......_ r:-. "'1 __ow;: . ,.., <br />X"\ ':,:(; , .. '."'..~ .""..... =",,-,'- eJ".::" . - - ". "'. .,. <br />t.{~...;:,-." ,. :....;.-r~.:... ".- r. J:t , e'. t' .f, . _~. . ~.:: -. _ ; v . <br />I -,,:,'r.;:,~~ ' . :....~...-e -t.:.;. - ''"'b-'. . :.,. ". __ . <br /> <br />~~'"(::..,~. ...r..'_'_ a~p'~:"':~' :&'''~~';'~~~'''o:~~;-....~r ~ ~ ~,~.~ ~i," ~~: <br />,.' A~ .... ~ J:-,O-: ..': ~ 'Q~...~'~;,~ '(6 1..... -.. .'! . ; .~~. -.-' <br />. #:: ~ . - . ,. _'" 0.., '.' .. (.;,. <br />.~"4I.P'"~"'. ":'!...~H .~~"~>IL '11:--r. .--....;,. _..,1,". c;.1~ .,-..._~ <br />.~~~ ~ ....::0 -~- 1:) -J'~' ~ r~:_' . - . .- . <br />J;#"~'~'~-'; "_~'C,/ ..'- ,-.~-.,;:;..;~..~.. <br />~ ~~.- ~ ;'-<' ~.'~'.'.:":.~'.: .~.~. e..~' ~ ~'~~.I;.~ . <br />.- ~~"'~~. "~' · .'-~.~ ~~.~~.-r..v.' . ';i..'" Co- <br />:....~~~~~.::.... - ~";=',;:.:~~~-'-~. ','..:,,;' - . ..-~ r~>' '..~ <br />, ~~'4"l ~~~~(O.'< (o. ,,' " - ."': '. - . ~.:. ' l' .. <br />;!...~r-~"(~.-t ..~.,~ . ,. : .....~:_ ;--.J'" yo 'c,_ ~ ." .. <br />"t',~""'lr."f~ '. ~l. "".;. (1',) _ t". . (; ',;.' 'Q G"'''?> . .~ <br />~&~~~':. .... _ "~.. _ ~_. _."._ ,.,.. ~ 3.~. ... <br />~~~~.. -', ",:).~.." ~-i....3J' ..,: " --:....-.1' ~~ ..: <br />r:I.. .. 1it= .e ~... \.!J ........: ~.. ...~./;.; .1!..- ~. ~ . .."., ~, <br />~.~. _~~_.~o '_ - .. _ -c.~.':.:..;;G >._,,,-'~.. . '. ;:,- <br />"!'- :..,...::. ~~..,.:. ",' ....... ....-,-,..;.. j - 4.;,- .~ 'II -., .,~ ~ <br />~.;.~ ~ . '. ."..-^ - 4" ."9:-i: 4.'.t, ": -<3<:1 ~ Go -::. a <br />. '." ... po......o.." ~ ':'."oJ..' -.....' I.l~~:_ ..:" ..~ _..- ,:.. <br />-. . C p' ~. - - ..~"6~__=:"'l'. ::,..e .....: ~>~., "",. oJ" f, ~,~, <br />..-~.~ ',' . .~ ~-..:'~:: ..;..~\~ :e--..;~!'::~::"\.: .~s - '-1.' .... ".0... <br />..,...'......'. .. 0"'" ....J"".,::.. ~"~.."N- i- .~.~ _,:"~e.-"" }'.'. ~. ", <br />. .. .....- Q..... :-. . -. _ ""' - .4r 4., <.";J ..... .. <br />~ - . . <" .. "....- ~ ' ~ - ~ ." ~ "f) . '-. <br />~ - '/;.-~' U"~; _;'" .." ~. . rai".- '-.".' - _ - ~ -. '" <br />". ~.' \). - '. .. ...., . ..' :.( ....., :1.. . -.... '3 <br />-.. ~ ~. ..,~ 0 -:~. : ."..,. _ . . ': . ~ :. '"'a ': _ . ~. . . . <br />~ :0_...- .~., ~.- - ~ _.rJ f \!.! ~.'~" .. <br />G ~ ~_,. -- - ~ .... '" . . ';'. ~ ::}. '. . <br /> <br />... "';:' ~. '. C!) ? G ~ _ .. W'.-"! "" <br />,~ :; t.;;...... N c.. ~ \- ~-....... '" "f)'. <br /> <br /> <br />FIGURE 5.-Histology of striped bass livers (a) from a control fish and (b) from a fish fed selenium-laden red <br />shiners. FV = fat vacuole; GV = glycogen vacuole; N = nucleus; ~ = pancreatic tissue with zymogen granules; bars <br />= 20 p.m. In (b), the hepatocyte cytoplasm is granular because of glycogen devacuolization, and the nuclei are <br />swollen. <br /> <br />terial). In other instances, proximal tubule epithe- <br />lium exhibited hydropic vacuolation, but in such <br />cases, the epithelial nuclei were not swollen. De- <br />generated tubules, lymphocytic infiltration, and <br />acidophilic tubule inclusions were observed in <br />some tissue sections. The trunk kidney of treat- <br />ment striped bass had normal glomeruli, and dis- <br /> <br />tal and collecting tubule structure was unaffected <br />by dietary treatment. Renal tubule damage similar <br />to that observed in treatment striped bass has been <br />documented in studies of other fish subjected to <br />excessive selenium exposure. Green sunfish from <br />selenium-contaminated Martin Lake and Belews <br />Lake have shown similar tubule damage in ad- <br />