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<br />I" <br />j <br /> <br />to be known. If so, use eqs. (21-22), with y <br />replaced by d = x - y, to estimate absolute <br />change and eqs. (27-28) to estimate relative <br />changes. To summarize, it may be possible to <br />estimate both population density and population <br />changes with reasonable precision, even in large <br />streams, if the sample size is large enough, but the <br />precision of the population density estimate may <br />be hard to obtain if M is difficult to measure. <br />Population density estimates from different <br />streams should, however, be compared with care. <br />In small streams there may be a possibility to <br />chose between all the three methods proposed <br />above. As stated above, a design with varying <br />section size is especially valuable if the 'natural' <br />biotope units are of a practicable size. Hankin <br />(1984) made the following general recommenda- <br />tions: <br /> <br />(1) If N is small (say 20 or less), neither the SRS <br />ratio estimation or the PPS with replacement <br />can be recommended. This leaves us to either <br />the SRS without use of auxiliary variable or <br />to the PPS without replacement. <br />(2) IfN is large (say 50 or more), the choice is less <br />clearcut. If N is very large (say 100 or more), <br />the SRS ratio estimation may be recom- <br />mended ifn is not too small (say 12 or more), <br />and the PPS with replacement if n is smaller. <br /> <br />As the sample size required usually is more than <br />12 and N often moderate or large, the SRS ratio <br />method appears to be useful, especially as the <br />result always can be directly compared with SRS <br />without auxiliary variable. As proposed above <br />concerning the sampling design of large streams, <br />a 'mixed' strategy may be useful even in small <br />streams, using the SRS ratio estimation for den- <br />sity or total stock and SRS without auxiliary <br />variable for population changes. <br /> <br />Electrofishing for other purposes <br /> <br />., When applied properly, longterm effects of elec- <br />trofishing on fish are small. The method is there- <br />fore suitable for catching fish to be kept alive for <br /> <br />35 <br /> <br />further experimentation. Non pulsed direct cur- <br />rent should then be used. <br />Electrofishing may be used in. situations other <br />than quantitative population estimates. In order <br />to ensure inclusion of a given fish species, it is <br />however, necessary to have certain degree of <br />knowledge of that species' habitat preferences <br />and selectivity when carrying out electrofishing. <br />When documenting the occurrence of different <br />fish species in a watercourse, it is essential that all <br />types of habitat (water velocities, depths, sub- <br />strates, etc.), are covered, while at the same time <br />making sure that those species are catchable with <br />an electrofishing apparatus. As Thymallus thymal- <br />Ius often is difficult to catch by electrofishing, it <br />may be necessary to include other equipment such <br />as sport fishing gear or nets. In such cases elec- <br />trofishing is carried out when water levels are <br />lowest. <br />In most studies it is necessary that specimens <br />are preserved for later examination. Experience <br />from Norway (as well as other places) has shown <br />that growth of pres molt salmon varies considera- <br />bly in different parts of the same river. It is <br />therefore essential that material for growth analy- <br />sis is collected from more than one location in the <br />river. The distance between collection stations <br />should never exceed ten kilometers. Repeated <br />electrofishing at short intervals should be avoided <br />because frequent electro shocking (< 3 months) <br />are likely to underestimate growth rates (Gatz <br />etal.,1986). <br />Dcnse populations of small, stationary trout or <br />other fish species are often found in streams and <br />small rivers. Fish required for release in other <br />rivers may be obtained by using electrofishing <br />equipment. Experiments conducted in Norway <br />have demonstrated that it is possible to catch <br />100- 200 trout per hour of a size between 5 and <br />20 centimeters in streams with dense fish popu- <br />lations. The advantage of this kind of fishing is <br />that large fish, capable of surviving, are caught at <br />the same time that an overcrowded trout popula- <br />tion is reduced. Fishing should be carried out at <br />low temperatures, in order to ensure that fish are <br />transported without high mortality. In order to <br />avoid spreading of undesirable fish species, dis- <br />