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<br />26 <br /> <br />Sampling design and stock assessment in streams <br /> <br />Introduction <br /> <br />So far we have been dealing with the question of <br />how to estimate the fish population in a closed <br />area in a stream or in the litoral zone of a lake. We <br />have also found methods well suited for this <br />purpose, at least for some species. <br />It is evident, however, that even if an exact <br />estimate were obtained from, say, pool 'A', this <br />figure is in most cases quite uninteresting. It <br />becomes useful first when we from this figure can <br />say something about the fish population in the <br />stream. It is also evident that if the population is <br />heavily varying from site to site, then we have to <br />study several such sites to make conclusions <br />about the whole population. So first we have to <br />define the population about which statements are <br />to be made, and then decide the size and number <br />of sites to be investigated, and finally find out how <br />to select these sites and how to calculate popula- <br />tion parameters from the result. <br />Too little attention has been paid to these <br />questions (Bohlin, 1981; Hankin, 1984), though <br />great savings may be the result if the field work is <br />well organized (or great losses if it is not). <br /> <br />The target area <br /> <br />The first crucial point in the planning of a <br />sampling programme is to define the target area <br />(sampling universe). The target area is the total <br />stream area (or in some cases stream length) that <br />one wishes to make statements about and in <br />which it is possible to carry out quantitative elec- <br />trofishing. In most applications the aim is to make <br />statements of the population, or to compare e.g. <br />'disturbed biotopes' with 'natural biotopes'. In the <br />first case the target area is the total fish-producing <br />area in a specific stream (minus the fish-producing <br />areas in which electrofishing is impossible). In the <br />latter case there are two target areas, of which the <br />first one is the total area of 'disturbed biotopes' in <br />the stream and the second the total area of <br />'natural biotopes'. In large streams it is important <br /> <br />to note that statements about the fish population <br />are limited to the population fraction in areas <br />(biotopes) where electrofishing is possible, so that <br />the target area may comprise only a minor <br />fraction of the total stream. <br />Even small streams may be to large for a total <br />survey, and one has to select the sampling areas <br />and determine the number and size of these. On <br />the other hand, if the target area is very small a <br />total survey may be practical, and no subsampling <br />is needed. <br /> <br /> <br />A <br /> <br />o RIFFLE BIOTOPE <br />EJ POOL BIOTOPE <br /> <br />34 <br />35 <br /> <br />36 <br /> <br />. 10 <br />'. 11 <br /> <br /> <br /> <br />r <br /> <br />I <br /> <br /><) <br />f <br />r <br /> <br />.., <br />( <br /> <br />Fig. 8. A stream divided into N sampling areas using (a) <br />units of equal length, (b) units of a size varying according to <br />biotope variation. A sample of units may be obtained using <br />simple random sampling in (a) and (b) or by proportional <br />probability sampling (b). The target area is the whole stream <br />except for the deepest part of the pool in the middle of the <br />stream. See also Fig. 9. <br /> <br />~ <br /> <br />( <br />