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, .. GENETIC VARIATION IN COLORADO SQUAWFISH 437 TABLE I.-Buffer conditions and tissues used to resolve loci in Colorado squawfish. Enzyme Gene or protein Protein number" symbol Bufferb TissueC Aconitate hydratase 4.2.1.3 ACOH-2 TEB M Aspanate aminotransferase 2.6.1.1 AAT-I TEB M AAT-2 TEB L Carboxylesterase 3.1.1.1 EST-I TC M Creatine kinase 2.7.3.2 CK-2 TEB M Enolase 4.2.1.11 ENO-2 TEB M Fumarate hydratase 4.2.1.2 FUMH-I TC M FUMH-2 TC M Il-Galactosidase 3.2.1.23 bGAL TC M Glucose-6-phosphate dehydrogenase 1.1.1.49 G6PDH-I TEB BE G6PDH-2 TEB BE Glucose-6-phosphate isomerase 5.3.1.9 GPI-I TEB M GPI-2 TEB M Glulamate-ammonia ligase 6.3.1.2 GLAL TEB BE Glyceraldehyde-3-phosphate dehydrogenase 1.2.1.12 GAPD-I TC, TEB M GAPD-2 TC,TEB M Glycerol-3-phosphate dehydrogenase 1.1.1.8 GJPDH TEB M Guanine deaminase 3.5.4.3 GDA-l TEB L GDA-2 TEB L Isocitrate dehydrogenase 1.1.1.42 IDH-2 TEB M L- Lactate dehydrogenase 1.1.1.27 LDH-I TC M LDH-2 TC M LDH-3 TC,TEB L Lactoylglutathione lyase 4.4.1.5 LGL TEB M Lens prolein LENS TEB BE or lens Malate dehydrogenase 1.1.1.37 MDH-I TEB M MDH-2 TEB M Malic enzyme 1.1.1.40 MDHP-I TEB M Mannose-6-phosphate isomerase 5.3.1.8 MPI TEB M Muscle protein MP-I TEB M MP-2 TEB M Nucleoside-triphosphate pyrophosphatase 3.6.1.19 NTP TEB M Peptidase (LGG)d 3.4.11 PEPB TEB M PEPS TEB M Phosphoglucomutase 5.4.2.2 PGM-l TMAL M PGM-2 TMAL M Phosphogluconate dehydrogenase 1.1.1.44 PGDH TEB M Phosphoglycerate mutase 5.4.2.1 e PGAM-2 TEB M Plasma protein PLP TEB BE Pyruvate kinase 2.7.1.40 PK-l TEB BE PK-2 TEB M Superoxide dismutase 1.15.1.1 SOD-l TEB M Triose-phosphale isomerase 5.3.1.1 TPI-I RGWY BE TPI-2 TEB M a Enzyme numbers follow the recommendations of [UBNC (1984). b Buffer systems: tris-EDTA-borate at pH 8.0 (TEB) and tris-<itrate at pH 7.0 (TC), both of Siciliano and Shaw (1976); tris- maleate at pH 7.4 (TMAL) of Harris and Hopkinson (1977); and Ridgway buffer at pH 8.5 (RGWY) of Ridgway et al. (1970). C Tissue extracts were muscle (M), liver (L), or brain plus eye (BE). d Optimal peplidase substrate, LGG = leucyl-glycyl-glycine. e Formerly 2.7.5.3. Results and Discussion ecules do not form in vi YO (Morizot and Siciliano 1983). Liver-specific LDH-3 variants were tetra- mers. Heterozygotes for PEPS were characteris- tically diffuse and consistent with the hexameric subunit structure examined by Frick (1983). Table 2 presents the allele frequencies for these poly- morphic loci. Three allozymes were observed at two loci, EST- 1* and GPI-2*. Not all alleles were shared among populations. The Green River and Dexter 1981 At least 9 of the 44 loci scored were polymor- phic. Heterozygotes for GPI-l, GPI-2, TPI-l, and TPI-2 allozymes were consistent with expecta- tions for dimeric enzymes. Enzymes exhibiting monomeric heterozygote patterns included MDH-2, EST-I, and bGAL. Although MDH-2 is a dimeric enzyme, in Colorado squawfish and oth- er fish species it appears that heterodimeric mol-