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<br />, '"
<br />
<br />that the fish can be collected by this method without
<br />altering several ofthese values. Blood lactate levels are
<br />elevated after electroshocking, return to pre-stressed
<br />levels in 3 h, but then continue to decline.
<br />
<br />References
<br />
<br />Black, E.C. 1955. Blood levels of hemoglobin and lactic acid in
<br />some freshwater fishes following exercise. J. Fish. Res.
<br />Board Can. 12(6):917-929.
<br />Bilinski, E., and R.E. Jonas. 1972. Oxidation of lactate to car-
<br />bon dioxide by rainbow trout (Salmo gairdneri) tissues.
<br />J. Fish. Res, Board Can. 29(10):1467-1471.
<br />Bouck, G.R 1972. Effects of diurnal hypoxia on electrophoretic
<br />protein fractions and other health parameters of rock bass
<br />(Ambloplites rupestrisJ. Trans. Am, Fish, Soc. 101(3):488-
<br />493,
<br />Caillouet, C.W. 1968. Lactic acidosis in channel catfish. J. Fish.
<br />Res. Board Can. 25(1):15-23.
<br />Dean, J.M., and C.J. Goodnight. 1964. A comparative study of
<br />carbohydrate metabolism in fish as affected by temperature
<br />and exercise. Physiol. Zool. 37(3):280-299.
<br />Denyes, H.A., and J.M. Joseph. 1956. Relationship between
<br />temperature and blood oxygen in the largemouth bass.
<br />J. Wildl. Manage. 29(2):56-64.
<br />Fraser, J,M., and F. W. Beamish. 1969. Blood lactic acid con-
<br />centrations in brook trout (Salvelinus fontinalis) planted by
<br />
<br />",.
<br />
<br />air drop, Trans. Am, Fish. Soc. 98(2):263-367
<br />Leivestad, H., H. Anderson, and P.F, Scholander. 1957.
<br />Physiological response to air exposure in codfish. Science
<br />126(2):505.
<br />Miles, H.M" S.M. Laehner, D.T. Michaud, and S.L. Salivar.
<br />1974. Physiological response of hatchery muskellunge
<br />(Esox masquinongy) to handling. Trans. Am. Fish, Soc.
<br />103(2):336-341.
<br />Schreck, C.B., RA. Whaley, M.L. Bass, O.E. Maughan, and
<br />M. Solazzi. 1976. Physiological reponses of rainbow trout
<br />(Salmo gairdneri) to electroshock. J. Fish. Res, Board Can.
<br />33( 1 ):76-84.
<br />Smitherman, RO. 1965. No title given. Pages 201-202 in 115
<br />Biennial Report, Louisiana Wildlife and Fisheries Commis-
<br />SlOn.
<br />Sterling, C. 1966. Biochemistry laboratory techniques. John
<br />Wiley & Sons Co., N. Y. 20 pp.
<br />Steucke, E.W" and C.R Atherton. 1965, Use of microhemato-
<br />crit values to sex largemouth bass. Prog. Fish-Cult.
<br />27(2):87 -90.
<br />Stevens, E.D. 1968. The effect of exercise on the distribution of
<br />blood to various organs in rainbow trout. Compo Biochem.
<br />Physiol. 25(2):615-625.
<br />Zar, J.H. 1974, Biostatistical analysis. Prentice Hall, N.Y.
<br />620 pp.
<br />
<br />Accepted 7 July 1978
<br />
<br />Improved Method for Collecting and Transporting
<br />Young American Shad
<br />
<br />This note reports an efficient method of collecting and
<br />transporting young American shad (Alasa sapidissima).
<br />The seining, handling, and transporting of recently
<br />metamorphosed shad are difficult and, until recently,
<br />resulted in high mortality. Attempts to use the method
<br />of Chittenden (1971) for seining shad, however, resulted
<br />in about 80-90% of the shad being too highly stressed to
<br />transport. Excessive stressing was prevalent when seine
<br />hauls contained several hundred shad together with
<br />numerous young striped bass (Marone saxatilis). We also
<br />found that Chittenden's transport system (a 550-liter
<br />baffled tank mounted on a vehicle) was complex and
<br />required too much handling of the shad. The present
<br />paper describes seining modifications which made pos-
<br />sible the live transport of 100% of the shad seined, and a
<br />transport system which reduces handling.
<br />
<br />150
<br />
<br />Shad were collected in fresh water from the Sac-
<br />ramento River near Rio Vista, about 128 km northeast
<br />of San .Francisco, California, with a beach seine 30.5 m
<br />long and 2.4 m deep (mesh size, 3.2 mm). The fish were
<br />concentrated in a small pocket of the net, which was
<br />quickly lifted and then submerged in a tub containing
<br />130 liters of 4% NaCl solution. Shad were then trans-
<br />ferred from the tub with a scoop cut from a 3.8-liter
<br />plastic bottle (Chittenden 1971) to Styrofoam chests
<br />containing the same brine solution. All chests were
<br />identical except that the interiors of some were white
<br />and those of others were dark gray. During transport an
<br />oxygen bottle with regulator was used to trickle oxygen
<br />into the covered chests. To evaluate the importance of
<br />saline solutions, shad were also handled and trans-
<br />ported in white chests with fresh water.
<br />
<br />THE PROGRESSIVE FISH-CULTURlST
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