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<br />, ... <br /> <br />d. <br />11. <br />Ie <br /> <br />is <br /> <br />~r <br /> <br />e <br />11 <br />Lt <br /> <br />y <br />s <br /> <br />CT <br />j <br /> <br />:l <br /> <br />. , <br />,/ <br /> <br />1 <br /> <br />SHOltT PAPERS AND NOTES <br /> <br />90 <br /> <br />80 <br /> <br />70 <br /> <br />60 <br />en <br />o <br />~~O <br />U <br />III <br />~40 <br />III <br />~ <br />i=30 <br /> <br />I ,I <br /> <br />>- <br />~20 <br />> <br />o \, <br />~IO <br />a: <br /> <br />I <br /> <br />o <br /> <br />-'-- <br /> <br />20 40 60 80 100 120 140 160 180 200 <br />ENERGY DENSITY (MILLIJOULES/C C.) <br /> <br />... <br /> <br />FIGURE 2.-Relation between average recovery time <br />and energy I density of shocked common shiners. Two <br />standard deviations of the mean are plotted in each <br />direction from the mean. <br /> <br />stressed the importance of voltage or voltage <br />drop in causing responses of fish to electrical <br />current. Past research has shown that fishes <br />go through a series of responses as the voltage <br />is increased, one of these stages being complete <br />immobilization or galvanonarcosis. The pres- <br />ent study is concerned with the galvanonarcosis <br />stage. Our results have shown the importance <br />of current and time in addition to voltage (i.e., <br />energy). However, with time held constant, <br />it can be shown that energy density is directly <br />proportional to the square of the voltage. The <br />energy density passing through a fish could <br />theoretically be calculated as: <br />. eXixT <br />Energy DenSIty = V IfF' h <br />o ume 0 IS <br />If the resistance of the fish is considered to be <br />a constant, R, Ohm's Law gives the current <br />through a circuit with e voltage as e/R. The <br />above formula now becomes: <br />e2 X T <br />Energy Density = R X (Vol. of Fish) <br /> <br />Thus the energy density through the fish <br />would be directly proportional to the voltage <br />squared. In studies where the immediate re- <br />sponse of the fish is measured (i.e., where time <br />is. not a factor) the above formula becomes: <br /> <br />555 <br /> <br />. e2 <br />Power Density = R X (Vol. of Fish) <br /> <br />The present study suggests that power density <br />may be a more meaningful measure of the <br />electrical stimulus than simply voltage or volt- <br />age drop as other workers have used; and <br />under laboratory conditions where time can he <br />measured, energy density may even he more <br />useful. . <br /> <br />LITERATURE CITED <br /> <br />DENZER, H. W. 1956. Die Elektrofischerei. Handb. <br />Binnenfisch. Mittlerop, Ergansungsband zu Band <br />5, Lieferung 3. 233 p. <br />HALSBAND, E. 1967. Basic principles of electric <br />fishing, p. 57-64. In R. Vibert (ed.), Fishing <br />with electricity. Fishing News (Books) Ltd., <br />London. <br />HOLZER, W. 1931. Dber eine absolute Reizspannung <br />bei Fischen. Pfliigers Arch. ges. Physiol. 229: <br />153-172. . <br />LAMARQUE, P. 1967. Electrophysiology of fish sub. <br />ject to the action of an electric field, p. 65-92. <br />In R. Vibert (ed.), Fishing with electricity. Fish- <br />ing News (Books) Ltd., London. <br />PATTEN, B. G., AND C. C. GILLESPIE. 1966. The <br />Bureau of Commercial Fisheries Type IV electro- <br />fishing shocker-its characteristics and operation. <br />U. S. Fish Wildl. Servo Spec. Sci. Rep. Fish. 529. <br />/ 15 p. <br />VWERT, R. 1963. Neurophysiology of electric fish. <br />ing. Trans. Amer. Fish. Soc. 92: 265-275. <br /> <br />WILLIAM J. ADAMS <br /> <br />DAVID J. BEHMER <br /> <br />WARREN O. WEINGARTEN <br /> <br />Lake Superior State College <br />Sault Ste. Marie, Michigan 49783 <br /> <br />A Simplified Device for Metering <br />Chemicals in Intermittent-flow <br />Bioassays <br /> <br />Many types of chemical metering devices <br />for use in intermittent-flow bioassays or other <br />tests have been described (Mount and W arner~ <br />1965; Mount and Brungs, 1967; Burke and <br />Ferguson, 1968). Intermittent-flow systems <br />have several- advantages over static systems in <br />bioassays, but experimental failures may re- <br />sult from the malfunction of moving parts <br />or fluctuations in flow rates. Complex meter- <br />ing devices require considerable time for <br />maintenance of proper concentrations. <br />Accurate delivery is essential when studying <br /> <br />- <br />A <br /> <br /> <br />I <br />l <br />J <br />i <br />I <br />J <br />J <br />