<br />reports of the results of the test should include
<br />the following information either directly or by
<br />reference to existing publications:
<br />l4.1.1 Name of test, investigator, and labo-
<br />ratory, and date test was begun;
<br />14.1.2 A detailed description of the toxicant,
<br />including its source, lot number, composition
<br />(identity and concentration of major ingredi-
<br />ents and major impurities), known physical and
<br />chemical properties, and identity and concen-
<br />tration of any solvents or other additives used;
<br />14.1.3 The source of the dilution water, its'
<br />chemical characteristics, and a description of
<br />any pretreatment;
<br />14.1.4 Detailed information about the test
<br />organisms, including scientific name and how
<br />verified (and strain for salmonids when appro-
<br />priate), weight (wet, blotted dry), length (stand-
<br />ard length for fish), age, life stage, source,
<br />history, observed diseases, treatments, accli-
<br />mation procedure, and fpod used;
<br />14.1.5 A description of the experimental de-
<br />sign and the test chambers, the depth and vol-
<br />ume of solution in the chambers, the way the
<br />test was begun, the number of organisms per
<br />treatment, the loading, the lighting, and for
<br />flow-through tests a description of the metering
<br />system and the flow rate as volume additions
<br />per 24 h;
<br />14.1.6 A description of any aeration per-
<br />formed on test solutions before or during the
<br />test;
<br />14.1.7 Defmition ofthe criterion used to de-
<br />termine the effect and a summary of general
<br />observations on other effects or symptoms;
<br />
<br />(1) This practice is based in large part on "Methods
<br />for Acute Toxicity Tests with Fish, Macroinver-
<br />tebrates, and Amphibians" by the Committee
<br />on Methods for Toxicity Tests with Aquatic
<br />Organisms, 1975, EPA-660j3-75-009. U. S. En-
<br />vironmental Protection Agency, Corvallis, Ore.,
<br />61 pp.
<br />(2) For example, see: International Technical In-
<br />formation Institute, Toxic and Hazardous In-
<br />dustrial Chemical Safety Manual, Tokyo, Japan,
<br />1977, 591 pp.; Sax, N. I., Dangerous Properties
<br />of Industrial Materials, 3rd Ed., Van Nostrand
<br />Reinhold Co., New York, 1%8, 1251 pp.; Patty,
<br />F. A., ed. Industrial Hygiene and Toxicology,
<br />Vol II, 2nd Ed., Interscience, New York, 1963,
<br />pp.831-2377.
<br />(3) A controller for such a transition period has
<br />been described in Drummond, R. A, and W. F,
<br />
<br />~ffi~
<br />
<br />E 729
<br />
<br />14.1.8 Percentage of organisms that died or
<br />showed the effect in the control treatment and
<br />the concentration of the toxicant in the control
<br />treatment;
<br />14.1.9 For daphnids and midge larvae, the
<br />24 and 48-h, and for all other organisms, the
<br />24,48, and 96-h LC50 or EC50 values and their
<br />95 % confidence limits and the method used to
<br />calculate them and for flow-through tests
<br />enough other LC50 or EC50 values to defme
<br />the shape of the toxicity curve; the concentra-
<br />tion of toxicant that killed or affected no greater
<br />a percentage of the test organisms than did the
<br />control treatment; for formulations and com-
<br />mercial products, specify whether results are
<br />based on whole mixture or active ingredient
<br />and whether results are based on measured or
<br />unmeasured concentrations;
<br />l4.1.10 Methods used for, and the results
<br />(with standard deviation) of, all chemical anal-
<br />yses of water quality and toxicant concentra-
<br />tion, including validation studies and reagent
<br />blanks, and storage procedures used, if any;
<br />14.1.11 The average and range of the accli-
<br />mation temperature and test temperature;
<br />14.1.12 Anything unusual about the test, any
<br />deviation from these procedures, and any other
<br />relevant information.
<br />
<br />15. Precision
<br />
<br />15.1 The results of acute toxicity tests con-
<br />ducted in different laboratories using the same
<br />species, dilution water, toxicant, and proce-
<br />dures will usually differ by no more than a
<br />factor of two.
<br />
<br />REFERENCES
<br />
<br />Dawson. "An Inexpensive Method for Simula-
<br />ting Diel Pallerns of Lighting in the Labora-
<br />tory," Transactions of the American Fisheries
<br />Society, Vol 99, 1970, pp. 434-435.
<br />(4) Such a substrate has been described in Frem-
<br />ling, C. R., "Acute Toxicity of the Lampricide
<br />3- Trifluoromethyl-4-Nitrophenol (TFM) to
<br />Nymphs of Mayflies (HexageniJJ sp, )," InPeSti-
<br />gations in Fish Con/rol No. 58, U. S. Fish and
<br />Wildlife Service, Washington, D. C. 1915, 8 pp.
<br />(5) A suitable device has been described in Ne-
<br />beker, A. V" and Lemke, A. E. "Preliminary
<br />Studies on the Tolerance of Aquatic Insects to
<br />Heated Waters," Journal of the Kansas Ento-
<br />mological Society, Vol 41, 1968, pp.411-418,
<br />(6) For example, see: Abram, F. S. H~ QApparatm
<br />for Control of Poison Concentration in Toxicity
<br />StudieswithFish," Water Jksemdt, Vol1, 1913,
<br />
<br />17
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