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<br />reports of the results of the test should include <br />the following information either directly or by <br />reference to existing publications: <br />l4.1.1 Name of test, investigator, and labo- <br />ratory, and date test was begun; <br />14.1.2 A detailed description of the toxicant, <br />including its source, lot number, composition <br />(identity and concentration of major ingredi- <br />ents and major impurities), known physical and <br />chemical properties, and identity and concen- <br />tration of any solvents or other additives used; <br />14.1.3 The source of the dilution water, its' <br />chemical characteristics, and a description of <br />any pretreatment; <br />14.1.4 Detailed information about the test <br />organisms, including scientific name and how <br />verified (and strain for salmonids when appro- <br />priate), weight (wet, blotted dry), length (stand- <br />ard length for fish), age, life stage, source, <br />history, observed diseases, treatments, accli- <br />mation procedure, and fpod used; <br />14.1.5 A description of the experimental de- <br />sign and the test chambers, the depth and vol- <br />ume of solution in the chambers, the way the <br />test was begun, the number of organisms per <br />treatment, the loading, the lighting, and for <br />flow-through tests a description of the metering <br />system and the flow rate as volume additions <br />per 24 h; <br />14.1.6 A description of any aeration per- <br />formed on test solutions before or during the <br />test; <br />14.1.7 Defmition ofthe criterion used to de- <br />termine the effect and a summary of general <br />observations on other effects or symptoms; <br /> <br />(1) This practice is based in large part on "Methods <br />for Acute Toxicity Tests with Fish, Macroinver- <br />tebrates, and Amphibians" by the Committee <br />on Methods for Toxicity Tests with Aquatic <br />Organisms, 1975, EPA-660j3-75-009. U. S. En- <br />vironmental Protection Agency, Corvallis, Ore., <br />61 pp. <br />(2) For example, see: International Technical In- <br />formation Institute, Toxic and Hazardous In- <br />dustrial Chemical Safety Manual, Tokyo, Japan, <br />1977, 591 pp.; Sax, N. I., Dangerous Properties <br />of Industrial Materials, 3rd Ed., Van Nostrand <br />Reinhold Co., New York, 1%8, 1251 pp.; Patty, <br />F. A., ed. Industrial Hygiene and Toxicology, <br />Vol II, 2nd Ed., Interscience, New York, 1963, <br />pp.831-2377. <br />(3) A controller for such a transition period has <br />been described in Drummond, R. A, and W. F, <br /> <br />~ffi~ <br /> <br />E 729 <br /> <br />14.1.8 Percentage of organisms that died or <br />showed the effect in the control treatment and <br />the concentration of the toxicant in the control <br />treatment; <br />14.1.9 For daphnids and midge larvae, the <br />24 and 48-h, and for all other organisms, the <br />24,48, and 96-h LC50 or EC50 values and their <br />95 % confidence limits and the method used to <br />calculate them and for flow-through tests <br />enough other LC50 or EC50 values to defme <br />the shape of the toxicity curve; the concentra- <br />tion of toxicant that killed or affected no greater <br />a percentage of the test organisms than did the <br />control treatment; for formulations and com- <br />mercial products, specify whether results are <br />based on whole mixture or active ingredient <br />and whether results are based on measured or <br />unmeasured concentrations; <br />l4.1.10 Methods used for, and the results <br />(with standard deviation) of, all chemical anal- <br />yses of water quality and toxicant concentra- <br />tion, including validation studies and reagent <br />blanks, and storage procedures used, if any; <br />14.1.11 The average and range of the accli- <br />mation temperature and test temperature; <br />14.1.12 Anything unusual about the test, any <br />deviation from these procedures, and any other <br />relevant information. <br /> <br />15. Precision <br /> <br />15.1 The results of acute toxicity tests con- <br />ducted in different laboratories using the same <br />species, dilution water, toxicant, and proce- <br />dures will usually differ by no more than a <br />factor of two. <br /> <br />REFERENCES <br /> <br />Dawson. "An Inexpensive Method for Simula- <br />ting Diel Pallerns of Lighting in the Labora- <br />tory," Transactions of the American Fisheries <br />Society, Vol 99, 1970, pp. 434-435. <br />(4) Such a substrate has been described in Frem- <br />ling, C. R., "Acute Toxicity of the Lampricide <br />3- Trifluoromethyl-4-Nitrophenol (TFM) to <br />Nymphs of Mayflies (HexageniJJ sp, )," InPeSti- <br />gations in Fish Con/rol No. 58, U. S. Fish and <br />Wildlife Service, Washington, D. C. 1915, 8 pp. <br />(5) A suitable device has been described in Ne- <br />beker, A. V" and Lemke, A. E. "Preliminary <br />Studies on the Tolerance of Aquatic Insects to <br />Heated Waters," Journal of the Kansas Ento- <br />mological Society, Vol 41, 1968, pp.411-418, <br />(6) For example, see: Abram, F. S. H~ QApparatm <br />for Control of Poison Concentration in Toxicity <br />StudieswithFish," Water Jksemdt, Vol1, 1913, <br /> <br />17 <br />