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<br /> <br />Introduction <br /> <br />The Colorado squawfish Ptychocheilus lucius is a large piscivorous cyprinid once <br />abundant in major tributaries throughout the Colorado River basin. It has, however, been <br />extirpated throughout much of its historic range following conversion of riverine habitat to <br />artificial impoundments, construction of migratory barriers, and introduction of nonnative fishes <br />(Tyus 1991a). The largest remaining populations of Colorado squawfish exist in the Green River <br />subbasin (Tyus 1991a), which is the least impacted of the large tributaries of the Colorado River. <br />Although the adult population in the Green River subbasin appears stable and persistent (McAda <br />et al. 1994), survival of young fish in nursery habitats composed of low velocity shoreline <br />embayments and backwaters is variable (Tyus 1991a; Tyus and Haines 1991) and may be <br />influenced by upstream releases from Flaming Gorge Dam (Carlson and Muth 1989; Valdez and <br />Cowdell 1996). The closure of Flaming Gorge Dam on the Green River in 1962 and its <br />subsequent operation is one potential cause of variable recruitment (Carlson and Muth 1989; <br />U.S. Fish and Wildlife Service 1992). In 1992 the U.S. Fish and Wildlife Service issued a <br />Biological Opinion (U. S. Fish and Wildlife Service 1992) that recommended discharge pattems <br />from Flaming Gorge Dam that more closely mimic the pre-impoundment hydrograph of the <br />Green River. Because little was known about the effects of winter and spring flows on young <br />Colorado squawfish the recommendations for winter and spring flows were largely based on <br />knowledge of adult Colorado squawfish. In 1992 the U.S. Bureau of Reclamation initiated the <br />Five-Year Flaming Gorge Research Program (Crist and Williams 1994), which proposed to <br />evaluate biological and physical responses of the Green River ecosystem to recommended flows <br />and obtain information about the effects of winter and spring flows on endangered fishes. <br />Several studies in the Five- Year Flaming Gorge Research Program emphasized study of <br />effects of environmental conditions on young-of-year Colorado squawfish. One study (Valdez <br />and Cowdell1996) estimated overwinter survival of young- of-year Colorado squawfish by <br />measuring relative densities (individuals/l00-m2 seined) in autumn and the following spring <br />based on catch per unit effort (CPUE). This method assumed that fish were equally vulnerable <br />(i.e., same probability of capture) to seining in the autumn and spring and that the same <br /> <br />1 <br />