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<br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br /> <br />Study 1 <br /> <br />All fish died in study 1 by 25 days of exposure (Figure 4, Appendix 3). No fish were <br />available for measurement of growth or selenium residue analysis. The survival curves and <br />median time to death were significantly different among locations (Table 9). Median time to <br />death was shortest in fish fed zooplankton from S 1 and longest in SR and NR. <br /> <br />Study 2 <br /> <br />The majority of fish died in study 2 by 12 days of exposure, including fish fed <br />invertebrates from the SI reference site (Figure 5, Appendix 4). The survival curve and <br />median time to death for larvae fed zooplankton from S5 was significantly less than the other <br />five locations, which were not different from each other (Table 9). Concentrations of <br />selenium in zooplankton ranged from 2.5 I-Lg/g at SI to 911-Lg/g at SR (Table 5). Whole- <br />body residues of selenium in fish ranged from 8.7 to 94 I-Lg/g (Table to). Fish fed <br />invertebrates from the Sl reference site contained 14.3 I-Lg/g (Table to). The whole-body <br />concentrations of selenium in larvae from study 2 were somewhat variable, as shown by the <br />standard error, due in part to the very small-sized samples available (0.1 to 0.5 mg dry <br />weight) and the imprecision of weighing submilligram samples. <br /> <br />Study 3 <br /> <br />Survival was 100% in all treatments through 9 days of exposure, but almost 50% <br />mortality or more occurred in the following 7 days (Figure 6, Appendix 5). The survival <br />curves and median time to death were not different between locations (Table 9). <br />Concentrations of selenium in zooplankton were 2.3 I-Lg/g in SI, 50 I-Lg/g in NR, and 87 I-Lg/g <br />in SR (Table 5). Whole-body residues of selenium in fish followed the same order of <br />progression as in invertebrates and were 3.7 I-Lg/ g in the S 1 treatment, 33 I-Lg/ g in the NR <br />treatment, and 39 I-Lg/ g in the SR treatment (Table 10). Fish length, weight, and condition <br />factor were not different between groups (Table 11). <br /> <br />Study 4 <br /> <br />Survival was 100% through 7 days in the S3 and S4 treatments, but only through 5 <br />days in the S5 treatment (Figure 7, Appendix 6). By day 20, survival was decreased to 22- <br />27 % . The survival curves and median time to death were not different between locations <br />(Table 9). Selenium concentrations in zooplankton ranged from 2.4 I-Lg/g in S4 to 12 I-Lg/g in <br />S5 (Table 5). Similar to study 3, whole-body residues in fish followed the same order as in <br />zooplankton and were lowest in treatment S4 (3.6 I-Lg/g), intermediate in S3 (3.9 I-Lg/g), and <br />highest in S5 (5.9 I-Lg/g) (Table to). Fish weight, but not length or condition factor, was <br />significantly reduced in larvae fed zooplankton from S5 (Table 11). <br /> <br />28 <br />