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7/14/2009 5:01:47 PM
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UCREFRP
UCREFRP Catalog Number
8088
Author
Horn, M. J., P. C. Marsh, G. Mueller and T. Burke
Title
Predation By Odonate Nymphs On Larval Razorback Suckers (
USFW Year
1994
Copyright Material
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372 <br />The Southwestern N¢tur¢list <br />Tnst.E 1-Numbers and total lengths (mm, Jf ± <br />SD) of larval razorback sucker remaining at the end <br />of two separate experiments totalling six experimental <br />exposures to odonate predators and four control setups <br />with no predators. Initial numbers of razorback sucker <br />were 100 in each setup far Experiment 1 and 75 in <br />each setup for Experiment 2. <br />Survivors <br />Experiment, Number <br />predator (percent) Total length <br />Experiment 1 <br />Damselfly 23 12.8 ± 0.7 <br />Damselfly 14 12.9 ± 0.5 <br />Dragonfly 28 12.9 ± 0.8 <br />Dragonfly 19 13.1 ± 0.5 <br />None 79 13.2 ± 0.7 <br />None 96 13.1 ± 0.8 <br />Experiment 2 <br />Damselfly 37 (49) 18.1 ± 2.0 <br />Dragonfly 33 (44) 17.2 ± 2.2 <br />None 64 (85) 17.1 ± 1.5 <br />None 59 (79) 17.8 ± 2.4 <br />Mq~ntnr c nND METHODS-Two experiments, the <br />first testing predation by odonate nymphs on 12-day- <br />old razorback sucker larvae and the second on 35-day- <br />old fish, were performed in continuously aerated, 40-1 <br />aquaria under a 12L:12D photoperiod. Odonate <br />nymphs were collected at night by light traps 1 to 3 <br />days prior to experiments. Larval suckers were from <br />eggs fertilized at Lake Mohave, incubated and hatched <br />at Arizona State University, and returned to lake back- <br />waters for growout. <br />Experiment 1 used six aquaria, each containing 35 <br />1 of unfiltered Lake Mohave water. Two aquaria con- <br />tained fish plus damselfly nymphs, two had fish plus <br />odonate nymphs, and two served as controls (fish only). <br />Water temperature was maintained at ca. 19°C. <br />One-hundred, 12-day-old larval razorback suckers <br />(mean total length [TL] = 11.8 ± 0.7 [SD] mm, n = <br />12) were placed in each aquarium on 10 March 1992 <br />and acclimated for 24 h; fish that died during this <br />period were replaced. Larval razorback sucker were <br />fed newly hatched brine shrimp nauplii (Artemis) (AU- <br />RUM Aquaculture) ad libitum before introduction into <br />aquaria and again mid-way through the 7-day exper- <br />iment. At 1000 h on 11 March 1992 (day 1), 20 dam- <br />selfly or dragonfly nymphs were added to each aquar- <br />ium. The experiment ran un-monitored until 18 March <br />1992 (day 8) when and all surviving odonates and fish <br />larvae were preserved in 5% buffered formalin. <br />The second experiment was conducted using 35-day- <br />old razorback sucker larvae (initial length not mea- <br /> <br />vol. 39, no. 4 <br />sated; estimated mean 14 to 15 mm TL, range 11 to <br />17 mm). Experimental design (photoperiod, duration, <br />water) was similar to experiment 1 with two excep- <br />tions: (1) treatments were not replicated and (2) num- <br />ber of fish per aquarium was reduced from 100 to 75. <br />Water volume was reduced to 26 1 to keep fish density <br />(2.9 fish/1) as in experiment 1. <br />Razorback sucker larvae were placed in aquaria on <br />7 April 1992 and allowed 24 h to acclimate. At 0845 h <br />on 11 April 1992 (day 1), 20 damselfly nymphs were <br />added to one aquarium and 20 dragonfly nymphs to <br />the other; two aquaria served as controls (fish only). <br />Water temperature was 23°C. Fish were fed ad libitum <br />twice, once at startup and again on day 6. The first <br />feeding was brine shrimp nauplii, the second of mixed <br />zooplankton filtered (80 micron) from Lake Mohave. <br />The experiment was terminated on 18 April 1992 (day <br />8); all fish larvae and odonates were preserved in 5% <br />buffered formalin. <br />Fish larvae were counted and measured (nearest 0.1 <br />mm TL). Tukey's multiple comparison test (Neter et <br />al., 1985) was used to compare mean number of sur- <br />viving razorback sucker larvae in each Experiment 1 <br />treatment. Survival in Experiment 2 was evaluated <br />using atwo-sample t-test (Snedecor and Cochran,1967). <br />R~sU1.TS-Larval razorback survivorship dif- <br />fered significantly (F = 39.4; df. = 2,3; P = <br />0.0075) among treatments in Experiment 1, with <br />greater survivorship in controls than in either <br />dragonfly or damselfly treatments (q = 10.44; df. <br />= 3,3; P = 0.01; q = 11.24, df. = 3,3; P = 0.009, <br />respectively). There was, however, no difference <br />between dragonfly and damselfly treatments (q <br />= 0.82; df. = 3,3; P > 0.5). <br />Because razorback larvae survival did not dif- <br />fer in dragonfly and damselfly treatments for Ex- <br />periment 1, results of the two treatments in Ex- <br />periment 2 were pooled to conduct a t-test. Mean <br />survival was significantly greater (t = 5.43, df. <br />= 2; P < 0.05) in controls (82%) than in pooled <br />odonate treatments (47%). <br />Average fish size among aquaria at the end of <br />Experiment 1 ranged from 12.8 to 13.2 mm TL, <br />and those in Experiment 2 from 17.1 to 18.1 mm <br />TL (Table 1); there was no difference (F = 1.01; <br />df. = 5,107; P > 0.5, Experiment 1; F = 0.84; <br />df. = 3,76; P > 0.5, Experiment 2) in final mean <br />TL of fish among aquaria within either experi- <br />ment. Average damselfly head width varied from <br />3.5±0.2mmto3.6±0.3mmandTL14.2± <br />1.1 mm to 14.4 ± 1.1 mm across all aquaria, <br />while average dragonfly head widths ranged from <br />5.8±0.8mm to 6.8±0.9mm and body TL <br />from15.2±2.4mmto19.4±3.5mm. <br />1 <br /> <br />
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