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<br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br />I <br /> <br />and Severn 1994b}. The mean number of cladocerans and copepods <br />from a backwater of the Gunnison River at the Escalante State <br />Wildlife Area, about 5 miles downstream from Delta, colorado, was <br />11 organisms per liter of water and the mean number from an open <br />water wetland was 25 organisms per liter (Cooper and Severn <br />1994c). Zooplankton (cladocerans and copepods) samples from a <br />bottomland (Old Charlie Wash) on the Ouray National Wildlife <br />Refuge contained a mean of 31 organisms per liter of water <br />(Cooper and Severn 1994d). Samples taken from the middle Green <br />River and a backwater on the refuge did not contain any <br />cladocerans or copepods. <br /> <br />C. Maior Limitinq Factor Affectinq Endanqered Colorado River <br />Fish Populations. The major limiting factor affecting the <br />populations of endangered Colorado River fishes is the lack of <br />successful recruitment (U.S. Fish and wildlife Service 1987, <br />1990a, 1990b, 1991). In the Lower Colorado River Basin, studies <br />have demonstrated that predation by non-native fishes and the <br />lack of food may be limiting the survival of larval and juvenile <br />endangered fishes (Marsh and Langhorst 1988; Papoulias and <br />Minckley 1990, Papoulias and Minckley 1992). Similar conclusions <br />have been reached for the endangered Colorado River fishes in the <br />upper basin (Miller et ale 1982; Maddux et ale 1993). <br /> <br />D. Relation of Food to Larval Fish Survival. Year-class <br />strength of marine fisheries is often related to the Match or <br />Mismatch Principle where environmental conditions such as water <br />temperature as well as the quality and quantity of food organisms <br />must match the hatching of larval fishes to realize high survival <br />(Hjort 1914, 1926). In other words, the density, size, time and <br />duration of availability of zooplankton must "match" the timing <br />of the swimup stage of fish larvae. When the yolk sac absorbed, <br />food organisms of the right size must be available to fish larvae <br />or they will starve. This principle was suggested by Marsh and <br />Langhorst (1988) for razorback sucker larvae in Lake Mohave and <br />documented for the razorback sucker larvae in the laboratory <br />(Papoulias and Minckley 1990). Razorback sucker larvae of about <br />10 mm total length were maintained in the laboratory at 18 C. <br />Unfed razorback larvae died in 10 to 30 days. Razorback larvae <br />must find food of the right size and density in 8 to 19 days to <br />survive. The "point of no return" when the fish died even though <br />sufficient food of the right size was available was between 19 <br />and 23 days for razorback sucker larvae. Papoulias and Minckley <br />reported that the quantity of food required for survival of the <br />razorback larvae was 20 brine shrimp nauplii per fish or high <br />mortality occurred. <br /> <br />E. Food Availabilitv for Larval Endanqered Fishes in the Upper <br />Colorado River Basin. The larvae of all endangered Colorado <br />River fishes feed on zooplankton early in life (U.S. Fish and <br />Wildlife Service 1987, 1990a, 1990b, 1991). The first ~oods of <br />larval razorback suckers in ponds were diatoms, detritus, algae, <br /> <br />29 <br />