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<br />channel and that fish species respond directly to available hydrologic and <br />physical habitat conditions. <br /> <br />Application of the methodology is comprised of several steps: 1) determining <br />the species distribution within the stream using literature reviews, field <br />observation, and consultation, 2) selecting a study reach using either a <br />critical reach or representative reach approach, 3) measuring physical <br />habitat and hydrologic characteristics in the reach using a multiple transect <br />approach, 4) determining species preference or use of streamflow variables <br />(depth, velocity and substrate), 5) using hydraulic simulation to determine <br />depth and velocity combinations at unobserved streamflows with respect to <br />substrate conditions, and 6) calculating weighted-useable-area (WUA) based on <br />the results from steps 4 and 5. _ <br /> <br />1. Soecies Distribution <br /> <br />The four rare fishes, historically located throughout much of the upper and <br />lower basins, have become restricted in distribution and abundance because of <br />obstructions to migration, changes in flow regime, and other physical and <br />biological factors (Miller et al. 1982; Jones and Tyus 1985; Tyus et al. <br />1987). To protect the remaining riverine habitat, the FWS Biological <br />Subcommittee completed a report to the UCRB Coordinating Committee identifying <br />"sensitive areas" of rare and endangered fish habitats in the Green, Colorado, <br />White and Yampa Rivers (Biological Subcommittee 1984). Rivers were segmented <br />and defined as "sensitive" by any of these four criteria: 1) endangered <br />species were identified or collected in the reach, 2) the reach was used as a <br />~pawning migration route, 3) the reach was confirmed or suspected as a <br />spawning area, or 4) the reach was considered a high density nursery area <br />(Biological Sub-Committee 1984). <br /> <br />Segment 3 within the Green River (FWS Segmentation Workshop, 8-12 December <br />1986) was identified as a sensitive reach because it is a high concentration <br />area and a suspected spawning area for adult Colorado squawfish, as well as a <br />nursery and distribution area for young-of-year and juvenile squawfish <br />(Biological Subcommittee 1984; FWS 1987; Tables 1 and 2). In addition it is a <br />concentration area and suspected spawning area for adult razorback suckers, <br />as well as a low concentration area for adult humpback chub. The biological <br />concerns within Segment 3 are as follows: 1) for Colorado sQuawfish: adult and <br />juvenile habitat year round, young-of-year nursery habitat from mid-July to <br />November 1, spawning habitat from mid-June to September, and migration routes <br />from mid-May to October (one month before and after spawning), 2) Razorback <br />sucker: adult habitat year round and spawning habitat from April to July, 3) <br />Humoback chub: adult habitat year round (FWS 1987; Table 2). <br /> <br />This segment, from river mile 248.1 to 345.0 (the Ouray Bridge upstream to the <br />confluence with the Yampa river), has six sub-segments within it based on <br />physical changes within the river channel. Sub-segment E from RM 326.8 to <br /> <br />3 <br />