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<br />I <br /> <br />, <br /> <br /> <br />I <br />I <br />I <br />,I <br />t <br />1 <br />I <br />I <br />I <br />I <br />j <br />1 <br />I <br />I <br />I <br />. <br /> <br />~. <br /> <br /> <br />I <br />, <br /> <br />a shocking boat or ground personnel started at the top of the backwater or <br />side channel and drove fish to the net by shocking or walking in and agitating <br />the water. This method enabled the field crew to quickly sample the backwater <br />and eliminated long-duration net sets. All non-target fish caught in trammel <br />nets were enumerated by species and returned to the river. <br /> <br />Rare Fish Species <br /> <br />All captured specimens of Colorado squawfish and roundtail chub were <br />anesthetized using MS-222 (200 mg/Liter of water), weighed, measured, and PIT <br />tagged. In addition, tissue samples were taken from all Colorado squawfish <br />and roundtail chub larger than 200 mm TL using standardized non-lethal, <br />aseptic techniques. Tissue samples were frozen in liquid nitrogen immediately <br />upon collection and later sent to Genetic Analysis in Smithville, Texas for <br />analysis. Five adult Colorado squawfish were surgically implanted with <br />Advanced Telemetry Systems radio tags. After implantation, the radio-tagged <br />fish were tracked by boat during subsequent sampling trips and by airplane. <br />Aerial tracking was conducted weekly during the presumed spawning season and <br />biweekly or monthly during the remainder of the year. Four radio tagged <br />Colorado squawfish were monitored intensively from the ground during another <br />study in the summer of 1993. <br /> <br />Compilation of Data <br /> <br />Because of the small number of recorded trammel net sets and limited <br />catch during July and October, trammel net data were lumped with electro- <br />fishing data to determine the total number of fish collected each year (total <br />catch). Electrofishing data were summarized as total catch per unit effort <br />(CPUE) per raft (i.e. total number of fish collected by all rafts combined in <br />a reach was divided by the total number of miles or hours shocked by all rafts <br />in that reach). All samples collected with a corresponding reading for total <br />time shocked were used in fish per hour calculations. All samples with <br />beginning and ending river miles were used in fish per mile calculations. <br />Only fish per mile data are presented in the text; fish per hour data are <br />given in Appendix I (Tables 9-11). In a few instances neither measure of <br />effort was recorded and catch per effort could not be calculated. Fish <br />collected in these samples were included in total catch but not used for CPUE <br />calculations. CPUE was not examined by time of year as each reach was sampled <br />only once and comparative data between trips was not available. Although <br />electrofishing cannot accurately sample small species (e.g., red shiners, <br />fathead minnows, and speckled dace), these fish were included in the analysis <br />of fish community composition because of the relatively high number of <br />specimens they represented. Actual numbers of these smaller species in the <br />main channel are probably much higher than indicated in the collection data. <br />Frequency of occurrence for all species was based on the percentage of <br />collections in which each species occurred. A collection is one <br />electrofishing sample (usually one mile of electrofishing), one trammel net <br />set, or one seine haul. <br /> <br />7 <br />