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<br />Discussion <br /> <br />Single exposure to homogeneous electric fields significantly reduced survival of <br />razorback sucker embryos in most treatments and growth of early larvae in all treatments. <br />Razorback sucker embryos and pre-swimup larvae in the Green and Yampa rivers would be <br />subjected to heterogenous electric fields possibly several times during their development <br />with electrofishing operations proven effective for collecting adults. Tyus and Karp (1990) <br />successfully captured adult razorback sucker by electrofishing over active spawning areas <br />at 2-3-d intervals. Razorback sucker eggs are demersal, and larvae remain hidden in the <br />substrate after hatching until they swimup and disperse from spawning areas (Snyder and <br />Muth 1990). Mean water temperatures during razorback sucker spawning in the Green and <br />Yampa rivers ranged 14-150C (Tyus and Karp 1989, 1990). At 150C, eggs hatch in <br />8.0-13.5 d postfertilization, and larvae swimup 13 d after hatching (Bozek et al. 1984; <br />Marsh 1985). Early life stages of razorback sucker concealed in the spawning substrate <br />(coarse sand to cobble) might be at least partially protected from electrofishing current. <br />However, Dwyer et al. (1993) demonstrated that eggs of Yellowstone cutthroat trout <br />Oncorhynchus clarki bouvieri placed in artificial redds and covered by about 15 cm of gravel <br />can be killed by the same level of electric current having a negative effect on survival of <br />eggs placed free in nylon-mesh baskets within a treatment chamber. <br />Sensitivity of razorback sucker embryos to electric fields decreased with advancing <br />developmental stage in all treatments, but mean survival at each developmental stage <br />beyond early epiboly remained significantly lower than that for controls except in the 30-Hz <br />and CPS treatments. Mean survival of embryos in the 60-Hz treatments was inversely <br />related to peak-voltage gradient. These observations are consistent with results of similar <br />studies on other fishes. Godfrey (1957) found that sensitivity of embryos of Atlantic salmon <br />Sa/mo safar and brook trout Sa/velinus fontinalis to 550-V, 1.7-A DC was low during the first <br />few hours of development (water hardening; pre-cleavage stages), high for stages from <br />early cleavage to end of epiboly, and low again thereafter. He also reported that mortality <br />increased with increasing exposure time (30-300 s) and field intensity (150 V, 0.6 A or 550 <br />V, 1.7 A). Newman and Stone (1992) evaluated the viability of 24 and 48-h <br />(postfertilization) walleye eggs exposed to quarter-sine wave, pulsed DC at 120 Hz, 400 V, <br />and 3 A. Eggs were put in nylon-mesh bags that were placed on typical walleye spawning <br />substrate in about 46-cm deep water and subjected to a single pass of an electrofishing <br />boat (minimum exposure duration that electrofishing in the wild would produce). Mortality <br />was 64% for the 24-h eggs and 56% for the 48-h eggs; mortality for shocked eggs at each <br />stage of development was significantly higher than that for eggs in controls. Incubation <br />temperatures and developmental state of eggs at treatment were not reported by Newman <br />and Stone, but walleye eggs incubated at 150C reached mid-cleavage in about 24 h after <br />fertilization and early epiboly in about 48 h (McElman and Balon 1979). Dwyer et al. (1993) <br />exposed eggs of rainbow trout O. mykiss at 2-26 d postfertilization for 10 s to a <br />homogenous, pulsed-DC (square-waveform) electric field of 240 Hz and 0.9-1.0 mean <br />V/cm; eggs hatched on day 28. Mean percent mortality for eggs treated on days 4-10 was <br />significantly greater than that in treatments on days 2 or 12-26 and controls; mortality on <br />days 2 or 12-26 was not significantly different from corresponding controls. Among <br />treatments, highest mortality occurred in eggs shocked on day 8, and differences were <br />significant. In another experiment, Dwyer et al. (1993) determined that mortality of <br />Yellowstone cutthroat trout eggs exposed 8 d after fertilization for 5, 10, or 20 s to CPS <br />current in a homogenous field increased with increasing voltage intensity ranging <br /> <br />12 <br />