My WebLink
|
Help
|
About
|
Sign Out
Home
Browse
Search
7717
CWCB
>
UCREFRP
>
Public
>
7717
Metadata
Thumbnails
Annotations
Entry Properties
Last modified
7/14/2009 5:02:30 PM
Creation date
5/20/2009 3:04:31 PM
Metadata
Fields
Template:
UCREFRP
UCREFRP Catalog Number
7717
Author
Rosenfeld, M. J.
Title
Genetic Analysis of the Colorado River Gila
USFW Year
n.d.
USFW - Doc Type
Non-fatal Sampling, Cell Culture, Chromosomal Analysis and Electrophoresis.
Copyright Material
NO
There are no annotations on this page.
Document management portal powered by Laserfiche WebLink 9 © 1998-2015
Laserfiche.
All rights reserved.
/
15
PDF
Print
Pages to print
Enter page numbers and/or page ranges separated by commas. For example, 1,3,5-12.
After downloading, print the document using a PDF reader (e.g. Adobe Reader).
Show annotations
View images
View plain text
<br />volume of distilled H20 and frozen at -80 oc. <br /> <br />I <br />Frozen fin samples were placed in an equal volume of cbld <br /> <br />PB8 at pH 7.2 and mechanically homogenized at 4 DC. <br /> <br />The res\..ll- <br /> <br />tant suspension was stored at -80 oC and thawed immediat~ly <br /> <br />before electrophoresis. <br /> <br />Fin homogenates were electrophoresed along with axial mus~le <br /> <br />samples ~aken earlier from the hatchery progeny of morpholo~i- <br /> <br />cally-distinct bony tail chubs from Lake Mojave, Arizona, ~nd <br /> <br />humpback chubs from Grand Junction, Colorado~ <br /> <br />as well <br /> <br />as rou!nd- <br /> <br />tail chubs captured near Grand Junction, Colorado. Muscle ti.sue <br />I <br /> <br />from hybrid fishes produced at the Dexter National Fish HatcHery <br /> <br />was also assayed. <br /> <br />8i:< fin clips from unidentified Gila caught: by <br /> <br />--t. <br /> <br />".,""""<.-f ' <br />listed in Table 1. Starch gel electrophoresis followed<-Alle~abrf.' <br /> <br />-..., <br /> <br />~ <br />.~ <br />, <br />.~ <br />~ <br />1 <br />~ <br /> <br />,. __..- ~ I <br />the Utah Division of Wildlife Resources at Cataract Canyon,.U~lilh, <br /> <br />were examined for lactate dehydrogenase. Assayed proteins iare <br /> <br />et al. (1977) ,while the acrylamide procedure 'fol1owed;~~mes <br /> <br />(1983). <br /> <br />" --' <br />,J <br /> <br />RESULTS <br /> <br />Morphometric analyses were done <br /> <br />on spreads consisting <br /> <br />of elongate chromosomes~ thus excluding the more contracted ones <br /> <br />common to <br /> <br />most ichthyological <br /> <br />studie!:;. <br /> <br />Mitotic arrestors <br /> <br />i <br />are necessary to obtain analyzable spreads but they also incr~ase <br /> <br />chromosomal contraction and, consequently, <br /> <br />their use result$ in <br /> <br />Hi (1 S t 1 \/ c.=, Hi ,01 1 1 F'r' h :i ~J h 1 y -- con d E' n '0; E' d c h r- 0 m (J ~; (J m e '=, ( S m i t h ~ 1 965) . <br /> <br />Such <br />
The URL can be used to link to this page
Your browser does not support the video tag.