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Last modified
7/14/2009 5:02:30 PM
Creation date
5/20/2009 3:04:31 PM
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UCREFRP
UCREFRP Catalog Number
7717
Author
Rosenfeld, M. J.
Title
Genetic Analysis of the Colorado River Gila
USFW Year
n.d.
USFW - Doc Type
Non-fatal Sampling, Cell Culture, Chromosomal Analysis and Electrophoresis.
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NO
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<br />necessary 1n order to assess the role of hybridization in <br /> <br />dc~termi n i ng the genet i c structure of the Colorado Ri ver Gi 1 a. <br /> <br />Genomic reassortment that would occur <br /> <br />in F2 and backcross <br /> <br />individuals could result in either retention of <br /> <br />hybrid LDH <br /> <br />patterns or in the restoration of parental phenotypes. Management <br /> <br />strategies for the Colorado River Gila are partly dependent on <br /> <br />the presence and extent of hybrid~zation. With regard to species <br /> <br />or population integrity, the consequences of hybridization would <br /> <br />be minimal if there were only F1 <br /> <br />idividuals that subsequently <br /> <br />never contributed to the gene pool. On the otherhand~ extensive <br /> <br />genetic mixing would ultimately lead to total introgession and <br /> <br />species or population breakdown. <br /> <br />These processes would be <br /> <br />distinguishable with a multiprotein electrophoretic scheme. As <br />listed in Table 1~ several proteins have been assayed with only <br /> <br />LDH yield assayable differences. However, there are severable <br /> <br />readily discernable enzymes and structural proteins that can be <br /> <br />examined and there is an ongoing program to do so in this <br /> <br />laboratory. Several more proteins are to be assayed on the study <br /> <br />material by the end of Spring 1985 (Table 2). Preferential <br /> <br />selection has been given to those proteins that tend to be <br /> <br />ta:.:onomically variable (G. B. Johnson~ 1974; M. S. Johnson and <br /> <br />Mickevich~ 1977; Sarich, .1977)~ since these may be more likely to <br /> <br />show interpopulational <br /> <br />electrophoretic migration differences. <br /> <br />Since all <br /> <br />'Methods and Materials' are <br /> <br />samples mentioned <br /> <br />in <br /> <br />currently frozen at -80 oC. <br /> <br />there is no need to obtain new <br /> <br />
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