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chub. statis~ically this diffe~ence is ve~y significant (t-test between ~oundtail chub and bony tail chub~ t=5.3~ df=78~ p<O.OOl). Fu~the~ diffe~entiation sepa~ating the bony tail chub f~om the humpback chub was done using NOR staining (Fig. 3). As many as th~ee diffe~ent ch~omosome pal~s were found to have stained ~egions. The humpback chub had th~ee ch~omosome pai~s with NORs. In cont~ast~ bony tail and ~oundtail chubs had simila~ o~ identical patte~ns~ with only two pai~s having stained ~egions. In a p~evious cont~act~ ch~omosomes were obtained directly f~om bonytail~ humpback and ~oundtail chubs~ instead of th~ough tissue cultu~e. The immediacy of ~esults with the fo~me~ methodology is desi~able; howeve~, most of the ~esultant ch~omo- some sp~eads a~e not suitable fo~ mo~phological analysis and high-~esolution sta~ning. In cont~ast, tissue cultu~e p~ovides a sou~ce of many suitable mitotic cells and the control ability-of , .~ ~ high-resolutiomn analysis. Until the present study a reasonable growth~ cell harvest and fixation yields chrom6somes usabl~ for procedu~e for using tissue culture in non-lethal population studies on fish has not been available. First~ only fin clips a~e requi~ed and these may be taken in the field. This tissue is often exti~pated fo~ ma~king pu~poses with little o~ no mo~tali- tv. Second~ the 'storage solution' provides nut~ients as well as maintains samples in an antibiotic environment~ allowing extended storage of collected material prIor to further labo~a- tory processing. Third~ cell cultures can be retained alive for