7259

• UCREFRP Catalog Number: 7259
• Author: Rosenfeld, M. J.
• Title: Preliminary Electrophoretic Study of the Colorado River Chub ‎(Pisces
• USFW Year: 1986.
• USFW - Doc Type: Cyprinidae, Genus
• Copyright Material: NO

at -20 Oc in a kitchen freezer prior to storage at -80 oC.

Liver samples were obtained from dissected specimens caught at

various places within the Colorado River drainage or from

hQtchery stock as outlined in Table 1.

Similar~y processed were

tui chubs, ~. bicolor, from Bishop Creek, Nevad2 (see Collection

G4 in Hubbs et al., 1974), and 50 Utah chubs, ~. atraria, from

five locations (Table 1).

All samples were placed in their own

labelled containers and stored under ultr?cold conditions.

Starch gel electrophoresis and histochemical staining procedures

followed Selander et al. (1971) and Harris and Hopkinson (1976),

with 3ss2yed proteins listed in Table 2.

All sample homogenates

were prepared in a pH 7.0 buffer solution.

Fin assays were

selected, in part, from those protein systems considered most

likely to be expressed in such tissue (D. Buth, pers. com.),

while the selections for liver were made, more or less randomly,

from proteins listed in Buth (1984).

Results were analysed as

single-individual genotypes using BIOSIS-1 <Version 2.0' a

program for the analysis of electrophoretic data (Swofford and

Selander, 1931), on a VAX computer.

RESULTS

~o differences were found in assays of fin clips.

In contrast,

sevpr~l differences were seen in co~parisons of liver protei~s.

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