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<br />Maximum species richness lines for metries ad- <br />dressing the number of species present were deter- <br />mined graphically using the historical and refer- <br />ence (1990) databases. Expected values for metries <br />dealing with percent composition of the catch and <br />for metries 11 and 12 were determined only from <br />the 1990 reference site database. Sampling tech- <br />niques in the historical database varied widely, <br />which could affect the relative abundance of species <br />collected. <br />Drainage area is considered an adequate meas- <br />urement of stream size and may be better than <br />stream order (Hughes and Omernik 1981). Drain- <br />age area was also chosen because it was the infor- <br />mation most readily available for streams within <br />this basin. Alternative measurements of stream <br />size, such as mean annual discharge or mean width, <br />were not available. <br />In determining the 5, 3, and 1 scoring for each <br />metric, the procedure described by Ohio Environ- <br />mental Protection Agency (1987) was followed. The <br />data were plotted against a loglO transfonnation of <br />drainage area. The graph was examined to deter- <br />mine if there was a positive relation with drainage <br />area and over what range of stream sizes this <br /> <br />CHRISTOPHER M. KAVANAUGH 17 <br /> <br />relation occurred. H a positive relation occurred <br />through any range of stream sizes, a line was drawn <br />to incorporate about 90% of the 1990 reference <br />sites. The area beneath this line was then trisected <br />equally. Where a positive relation was not found, <br />an alternative trisection method was used. For <br />these metries, a horizontal 5% and 95% line was <br />determined, and the area between them was tri- <br />sected. <br />Sixty-four fish species representing 17 families <br />were collected in 1990 (Table 7). Fifty-four species <br />were collected in 13 boom electrofishing stations, <br />49 species from 16 stream electrofishing stations, <br />and 30 species from 16 backpack electrofishing <br />stations. The boom electrofishing stations had a <br />higher percent composition (59.2%) of large fishes <br />(excludes minnow, darter, and other small fish <br />species) compared with the stream (18.7%) and <br />backpack (6.5%) stations. <br />The IHI scores for the 1990 reference sites <br />ranged from 20 to 56. The median score was 36, and <br />the mean was 37.5. Karr et al. (1986) considered <br />illI values less than 34 (out of a possible score of GO) <br />as representative of poor or very poor conditions. <br /> <br />Table 7. Fish species sampled at 45 reference sites in 1990a, occurring in the historical data base, and <br />reported in Underhill (1989). <br /> <br />Historical <br />Scientific name Common name data Underhill <br /> <br />Ichthyomyzon unicuspis Silver lamprey a X <br />Lampetra appendix American brook lamprey x x <br />Scaphirhyncus platorynclms Shovelnose sturgeon a X X <br />Polyodon spatlwla Paddlefish X X <br />Lepisosteus osseus Longnose gar X X <br />L platostomus Shortnose gar a X X <br />Amia calva Bowfin X x <br />Hiodon alosoides Goldeye a X X <br />H tergisus Mooneye a X X <br />Anguilla rostrata American eel a X X <br />Dorosoma cepedianum Gizzard shad a X X <br />Campostoma anomalum Central stoneroller a X X <br />C. oligolepis Largescale stoneroller a X X <br />Carassius auratus Goldfish X X <br />Cyprinella spiloptera Spotfin shiner a X X <br />Cyprinus carpio Common carp a X X <br />HybognatJws hankinsoni Brassy minnow a X X <br />Luxilus comutus Common shiner a X X <br />Macrhybopsis aestivalis Speckled chub X X <br />M. storeriana Silver chub X x <br />Margariscus margarita Pearl dace x X <br />Nocomis biguttatus Homyhead chub a x X <br />Notemigonus crysoleucas Golden shiner x x <br />Notropis anogenus Pugnose shiner x <br />