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<br />fishes in the region. This was to be accomplished by preparation of a manuscript (this [mal <br />report) for subsequent publication as a supplement to the 1990 guide. The supplement would <br />include updated descriptive information for the species covered in that guide, a comparable <br />descriptive species account for longnose sucker, an updated and expanded comparative <br />summary, and a new computer-interactive key or keys (program and files on diskette or compact <br />disk) to correct, update, and expand upon the existing printed keys. A secondary objective of the <br />project was to provide proof-of-concept for the effective application of a relatively new <br />taxonomic tool to larval-fish identification, the computer-interactive key. <br /> <br />Methods <br /> <br />Specimens Examined <br /> <br />Description of the larvae and early juveniles of longnose sucker was based on specially <br />assembled developmental series that were laboratory-reared in 1979 and 2001 from eastern- <br />slope, north-central Colorado populations and selected collections of wild early juveniles <br />(young-of-the-year) from the lower Gunnison River in 1993 and 1995. Ninety-eight (98) <br />specimens were examined and analyzed in detail, 57 specimens were cleared and stained with <br />alizarin red for examination of skeletal features, and still others were cursorily examined for <br />extremes in developmental state or pigmentation. Specimens were either fixed in 10% formalin <br />and subsequently preserved in phosphate-buffered 3% formalin or preserved directly in 95% <br />ethanol without formalin fixation. All specimens are maintained as part of the LFL Collection. <br />Specimens analyzed, examined, or illustrated in detail were individually cataloged as: <br />LFL# 6690 (1 specimen; 37.0 m.m TL, total length) - Collected 7 September 1993 from <br />Gunnison River kIn 96.1, Delta County, CO, by USFWS; formalin. <br />LFL# 6837 (1 specimen; 38.5 m.m TL) - Collected 7 September 1993 from Gunnison River km <br />83.7 by USFWS; formalin. <br />LFL# 26446 (1 specimen; 34.7 mm TL) - Collected 19 September 1995 from Gunnison River <br />kIn 55.7 by USFWS; formalin. <br />LFL# 67261-67263, 67265, 67274-67275, 67277-67278 (8 of 166 specimens from LFL# <br />26647; 21.1-38.0 m.m TL) - Collected 21 September 1995 from Gunnison River kIn <br />94.0 by USFWS; formalin. <br />LFL# 67264, 67273, 67276 (3 of 10 specimens from LFL# 26520; 25.1-30.6 mm TL)- <br />Collected 19 September 1995 from Gunnison River km 80.0 by USFWS; formalin. <br />LFL# 67266-67267 (2 of7 specimens from LFL# 6799; 42.5-46.0 mm TL) - Collected 7 <br />September 1993 from Gunnison River kIn 84.0 by USFWS; formalin. <br />LFL# 67268, 67272 (2 of 4 specimens from LFL# 6703; 32.8-48.8 mm TL) - Collected 7 <br />September 1993 from Gunnison River kIn 95.5 by USFWS; formalin. <br />LFL# 67269-67270 (2 of39 specimens from LFL# 6758; 46.2-50.2 mm TL) - Collected 7 <br />September 1993 from Gunnison River km 89.5 by USFWS; formalin. <br />LFL# 67271 (1 of 10 specimens from LFL# 6899; 42.8 mm TL) - Collected 7 September 1993 <br />from Gunnison River km 83.1 by USFWS; formalin. <br />LFL# 67220-67260 (41 specimens, 7.6-19.9 mm TL) - From developmental series that was <br />laboratory-reared in 1979 by LFL from Parvin Lake stock, Larimer County, CO; <br />formalin. <br /> <br />11 <br />