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<br />"- <br />Fish were subjected to pulsating direct current ob- <br />tained by conversion from an AC source. Fish were <br />placed in stalls between the electrodes in the water- <br />filled plastic aquariurn and the electrical system was <br />energized. Irnmediately after the desired duration of <br />exposure, the fish were placed in 94.6-liter aquariurns <br />with continuous water flow and aeration. The <br />aquariums were checked for dead fish at intervals of 1,3, <br />6, and 24 h after exposure and then daily for 7 days. <br />Control fish were treated similarly, except that the elec- <br />trical system was not energized. <br />Ten fish per test were subjected to pulse frequencies of <br />1.6,8.8, and 16.0/s while other electrical characteristics <br />remained constant. Each pulse frequency rate was <br />tested on fish exposed for 5, 15,30,45,60, 120, and 180 s. <br />All tests were replicated. Fish were exposed to an elec- <br />trical field only once (a fish surviving one test was never <br />used in another). <br />Darters ranged in length frorn 2.5 to 7.5 crn and <br />bluegills from 9.0 to 17.0 crn. Fish of similar size were <br />tested together. No significant differences in rnortality <br />between these size ranges were noted. <br /> <br />Resul ts and Discussion <br /> <br />Fishery biologists prefer to use direct rather than <br />alternating curr~nt for electrofishing because of the <br />electrotactic response. Pulsed direct current produces <br />even greater anodic attraction than does continuous di- <br />rect current (Collins et al. 1954; Miller 1962; Northrop <br />1967; Vincent 1971). Although optirnurn pulse rates for <br />electrotaxis of fish have not been clearly defined, Nor- <br />throp (1967) noted that low pulse rates (1 to lO/s) pro- <br />duce poor electrotaxis. Higher pulse rates (above 50/s) <br />tend to narcotize, damage, or kill fish and do not perrnit . <br />electrotaxis (Northrop 1961; Collins et al. 1954). <br />Mortality of the test fish increased with increases in <br />the electrical characteristics examined. Duration of ex- <br />posure appeared to be the factor most responsible for <br />death offish. Mortality was low when fish were exposed <br />for 15 s or less, but increased progressively with dura- <br />tion of exposure. On the basis of these data, mortality of <br />electroshocked fish should be negligible if fish are re- <br />moved frorn the electric field within 15 s after initial . <br />shock and if other electrical characteristics ernployed <br />are within the ranges studied. <br />Tirne required for fish to recover equilibriurn in- <br />creased with duration of exposure. The loss of equilib- <br />rium for even a short tirne could be of serious conse- <br />quence in a strearn, where lethargic fish would be easy <br />prey for predators. After the fish have been shocked, the <br />fish rernoved frorn a stream should be held in well-aer- <br />ated holding tanks until they recover equilibrium. The <br />length of time that fish should be held for full physiolog- <br />ical recovery is about 24 h (Schreck et al. 1976). How- <br />ever, since this amount of holding tirne is unreasonable <br /> <br />162 <br /> <br />for field. operations, releasing fish only after they have <br />fully recovered equilibriurn should decrease loss of fish <br />to predation. <br />OUf data showed relatively high mortality of fantail <br />darters and bluegills in the pulse frequency defined as <br />gi ving good electrotactic response (Figs. 1,2). However, <br />because the fish were held in alignrnent parallel to the <br />direction of the field, they were subjected to rnaximurn <br />voltage. At any other angle a fish would receive less <br />than maxirnurn voltage, and would be less likely to be <br />killed. In a stream, fish would be randomly located in an <br />electrical field, prirnarily aligned parallel to water cur- <br />rent and would be subjected to varying voltages. There- <br />fore, the percentage of fish killed directly by the shock <br />should be lower under stream conditions than under our <br />experimental conditions. <br /> <br />I <br />, <br />, <br /> <br />100 <br /> <br />90 <br /> <br /> <br />o - 1.6 pulses/s <br />c - 8.8 pulses/s <br />6 - 16.0 pulses/s <br /> <br />A <br /> <br />6 <br /> <br /> 80 <br /> 70 6 <br /> 60 <br />~ <br />.~ 50 <br />OJ <br />~ <br />0 <br />~40 <br /> 30 <br /> 20 <br /> 10 <br /> <br />30 60 120 180 <br />Duration of Exposure (s) <br />Fig. 1. The relation of pulse frequency to the effect of duration of <br />exposure on mortality offantail darters. Test run at 4.0 V/crn. <br /> <br />. <br />I <br /> <br />Our data are not directly applicable to a field situation <br />because of the enforced orientation of the fish parallel to <br />the electric field, the rectilinearity of the field induced <br />by the plate electrodes, and the confinement of the field <br />within the plastic aquarium. However, our results offer <br />guidance to biologists on the levels and patterns of pul- <br />sating direct current applicable to field collection situa- <br />tions. Preliminary sarnpling of a section of each stream <br /> <br />L, <br /> <br />THE PROGRESSIVE FISH-CULTURIST <br />