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<br /> <br /> <br />F_~~q'-.:~. ,:' ',' <br /> <br />(,? .~'~,_::: ~.::~t:~ . - , <br /> <br />109'37'30"' <br /> <br /> <br />~ <br /> <br />4O"OT3O" <br /> <br />o <br />I <br />o <br /> <br />109"22'30" <br /> <br /> <br />. ,8;'Waddelllaila:r~.S. May <br /> <br />1- <br />J <br /> <br />t <br /> <br />. EXPlANATION <br />... Muscle plugs-1M! Razocback Suckers <br />. Muscle plugs - . an:hiwd Razorback Sucker <br />. Muscle p1ug':'1iw Razorback Sucker hybrid <br />* Musa; piugs and tissue - other fish species <br /> <br /> <br />I <br />4 <br /> <br />4 <br />I <br /> <br />8 MIlES <br />I <br /> <br />I <br />8 KIlOMETERS . <br /> <br />fish is not desirable and in most cases notpenriitted, We also <br />evaluated the suitabilit}'of a nori-Iethal collectiotltechnique for <br />fish. muscle for deterinining seleniumandpossi6ly. other con- <br />t3niinantS. .. "" _'I;: <br /> <br /> <br />Collecti~nof Muscle Plugs from Live RazorbackSuckers <br /> <br />The studylirea arid Umple collection sites are identified in Figure I. <br />RazorbaCk Bar and Escalante Bar are approximately 20 Ian and 16 Ian <br />upstreanl'from the mouth of Ashley Creek, and Old Charlie Wash is <br />approximately 110 kmdownstream from the. mouth of .Ashley Creek. <br /> <br /> <br />Utah <br /> <br />Fig. 1. Portion of Green River <br />and tributaries comprising the <br />study area <br /> <br />All samples were collected April through June, 1992. Razorback suck- <br />ers. were captured by electroshocking and drift trammel net. Muscle <br />plugs were taken following protocol specified by Williamson (1992), <br />in which a separate 5-mm sterile biopsy punch was used to collect a <br />single plug Iromeachfish. The area of the fish being sampled was <br />cleaned withethyhlcohol and the punch 'was inserted, twisted, and <br />removed./The$lllDple. collecliolland' removal.. from .the punch was <br />assisted with the use of a scalpel,. as required.01lemuscle plug was <br />taken frolD. each live I3ZOrbacksucker fr:omthemiddorsal area about <br />1-2 cm from the dorsal fin. Muscle plugs were placed in chemically <br />clean vials, chilled with ice in the field, frozen the same day, and kept <br />frozen until samples were prepared for analysis. All collections of <br />muscle plug tissue and handling of endangered fish were done cooper- <br />atively with personnel from the Colorado River Fishery Project, Ver- <br />nal, Utah. <br />