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Last modified
7/14/2009 5:02:35 PM
Creation date
5/20/2009 1:38:26 PM
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UCREFRP
UCREFRP Catalog Number
9470
Author
Shiozawa, D. K., M. D. McKell, B. A. Miller and R. P. Evans.
Title
Genetic Assessment of four native fishes from the Colorado River drainages in western Colorado
USFW Year
2003.
USFW - Doc Type
the results of DNA analysis.
Copyright Material
NO
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<br />between individuals or populations. Thus no phylogenetic signal was present. We then <br />attempted to amplify several other nuclear genes, including GTH, but were unable to obtain <br />useful data. This problem occurred with all four species examined in the study. Therefore it was <br />not possible to generate nuclear based phylogenies. <br /> <br />Analvsis <br /> <br />Analysis of the resulting data sets included general maps and tables of haplotype frequencies and <br />the generation of F statistics and Nm values. F statistics were generated with Arlequin: A <br />software for population genetic data analysis, version 1.1 (Schneider et a1. 1997) as was the <br />matrix of significant Fst values. This program also generates Nm values which can be used to <br />estimate the number of migrants between populations per generation. However, this estimate <br />must be interpreted with caution since a strong historical component exists in the data. <br />Minimum evolutionary trees were generated by MEGA (Kumar et aI2001). These trees, based <br />on the Tamura-Nei (1993) index, which differentially weights distance estimates with base <br />position and transition-transversion probabilities, allow a visual comparison of relationships <br />between haplotypes. <br /> <br />26 <br />
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