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<br />between individuals or populations. Thus no phylogenetic signal was present. We then <br />attempted to amplify several other nuclear genes, including GTH, but were unable to obtain <br />useful data. This problem occurred with all four species examined in the study. Therefore it was <br />not possible to generate nuclear based phylogenies. <br /> <br />Analvsis <br /> <br />Analysis of the resulting data sets included general maps and tables of haplotype frequencies and <br />the generation of F statistics and Nm values. F statistics were generated with Arlequin: A <br />software for population genetic data analysis, version 1.1 (Schneider et a1. 1997) as was the <br />matrix of significant Fst values. This program also generates Nm values which can be used to <br />estimate the number of migrants between populations per generation. However, this estimate <br />must be interpreted with caution since a strong historical component exists in the data. <br />Minimum evolutionary trees were generated by MEGA (Kumar et aI2001). These trees, based <br />on the Tamura-Nei (1993) index, which differentially weights distance estimates with base <br />position and transition-transversion probabilities, allow a visual comparison of relationships <br />between haplotypes. <br /> <br />26 <br />