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<br />Methods and Results <br /> <br />Hatcheries <br /> <br />Willow Beach National Fish Hatchery, Arizona and Dexter NFH, New <br />Mexico and the Utah Fisheries Experiment Station, Logan, Utah are <br />propagating Colorado River fishes for the CRFP. Willow Beach has <br />been the primary producer of the fish and has been supplying the other <br />two hatcheries and laboratories with fry, fingerlings and future <br />broodstock. <br /> <br />Colorado squawfish - A group of 27 wild adult squawfish were held <br />at Willow Beach for broodstock. They were collected from the Yampa, <br />Green and Colorado Rivers from 1973 to 1979. Also, about 2,000 <br />squawfish were produced at the hatchery in 1974. They began to mature <br />in 1979 and about 500 were selected for broodstock. Of these, 100 <br />remain at Willow Beach; 140 are at Utah FES; 270 are at Dexter, and 16 <br />are at Utah State. Unfortunately, 14 of the wild squawfish held at <br />Willow Beach were stolen on November 27, 1980 and six of eight mature <br />females were lost. <br /> <br />Broodstock at Willow Beach are held in double 100 foot raceways <br />that are 8 ft. wide and 4 ft. deep. Several tons of gravel have been <br />placed in the upper 1/3 of some of the raceways where water can flow up <br />through the gravel. The gravel is sloped to provide depths from 0-75 cm <br />for the fish to spawn on. The water is from the Colorado River, unfiltered <br />and unsterilized. It is recirculated beginning in early spring to allow <br />it to gradually warm up to 700 F. over a period of months. This brings <br />on maturation of the fish about a month after the water temperature <br />reaches 68 to 700 F. <br /> <br />Spawning previously occurred over the gravel beds in 1974 and 1979. However, <br />1980 has turned out to be the best year on record. Three females were <br />observed releasing eggs on June 23 and 24 and produced an estimated <br />25,000 eggs. <br /> <br />Following the natural spawning, five of the wild broodstock and <br />10 domestic females were subjected to injections of carp pituitary to <br />induce spawning. The wild broodstock were released and allowed to <br />spawn on their own producing an estimated 40,000 eggs. In addition <br />approximately 1,000 eggs were hand stripped from one wild female. and <br />78,000 were stripped from the dom~stic broodstock. This was the first <br />time these methods have been used successfully on the Colorado squawfish. <br /> <br />Up to 95% of the wild eggs from natural spawning hatched success- <br />fully. But only 1,000-2,000 eggs hatched of those taken from the domestic <br />broodstock. Hatching usually occurred in 96 hours at 200 C. <br /> <br />112 <br />