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<br />.1 <br /> <br /> <br />};~~ <br />,~... <br />-( <br /> <br />by releasing imprinting chemicals (and control water) during the anticipated <br />spawning period. <br /> <br />Response of the imprinted fish to suitability of the attractant and various <br />habitats would be monitored by recaptures. <br /> <br />H5: Colorado squawfish and razorback suckers recognize odors from reproductive <br />byproducts and use them to locate specific sites for egg deposition. <br /> <br />Task: Determine if reproductive by-products can be used to attract fish in <br />lieu of using a synthetic chemical. <br /> <br />Methods: Obtain biological waste-products (egg cases, feces, et ) from <br />hatchery-reared fish, and place these in selected spawning locatio s. Monitor <br />the location by capturing fish during the spawning season. This co ld be done <br />in the presence or absence of other cues (HSOB or synthetic compou ds) <br />depending on study design. <br /> <br />Morphology <br /> <br />It is important to determine temporal development of functional I receptors <br />used in olfaction to determine the timing associated with imprinting. <br />Therefore it is necessary to determine the comparative functional ~orphology <br />of sensory organ development and their function. r <br /> <br />Also there are accessory organs involved with reproduction in i' th species: <br />In the Colorado squawfish, breeding tubercles are hard, horny structures that <br />may have a tactile function in breeding, and razorback sucker tubercles are <br />soft and fleshy organs that may be involved with secretions of at ractant <br />odors. <br /> <br />H6: Functional receptors used in olfaction develop at an early st ge in <br />Colorado squawfish and razorback sucker <br />Task: Determine the development of olfactory sensory organs <br /> <br />Methods: Obtain a series of ontogenic stages for both species tnd <br />determine structures microscopically. <br /> <br />Task: Determine neuroanatomy of sensory system: when do connections become <br />functional (i.e. "turned on"). I <br /> <br />Methods: Obtain a series of ontogenic stages for both species ~nd <br />determine structures microscopically. I <br /> <br />H7: Breeding tubercles are important reproductive organs that ma be involved <br />in attracting mates and influence the successful completion of s awning. <br /> <br />Task: Evaluate the role of tubercles in reproduction. <br /> <br />Methods: Obtain tissues from sexually-mature fish and examine these <br />structures microscopically to determine possible function. <br /> <br />:~ <br /> <br />I <br /> <br />~~J."" <br />':~y <br /> <br />;, <br />, <br />:,,~,:.; <br />i <br /> <br />.':"'1~~' <br /> <br />t <br /> <br />I <br /> <br /> <br />..~ <br /> <br />~~,~ <br /> <br />I.-......'..:.! <br /> <br /> <br /> <br /> <br />" <br /> <br />~ ., i <br /> <br />i;< <br />, <br />v! <br />.... <br /> <br />] <br />. , <br /> <br />';i~ <br /> <br />.--, <br />;-<>) <br /> <br />... I.......,'...:' <br />" <br /> <br />~,>~ <br />, ".~ <br /> <br />:~1~! <br /> <br /> <br />16 <br /> <br />