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<br />ng/g body weight} ranged from approximitely 2 x to 5 x (i.e., 200 to 500%) <br />that of basal levels observed in the early egg stages (1.6 to 4.2 ng/g body <br />weight). Concentration of the surges at the time of swim-up (11.8 to 14.9 <br />ng/g body weight) ranged from about 2 x to 4 x (i.e., 200 to 400%) that of <br />level observed in recently hatched yolk sac larvae (3.4 to 6.4 ng/g body <br />weight). We believe that these results represent a true increase in <br />thyroid concentrations because quality assurance tests indicated that <br />because results of the assay was reliable. Measured concentrations of <br />quality control samples were within the acceptable range of the actual <br />concentrations. Although the mean percent error and maximum error were <br />both high, thyroid surges were well above the range of these errors. The <br />mean percent error (:t 95% el) of duplicate samples was 8.2 :t 1.5%, and the <br />range was from 0.1 to 35.3%. well below the 200% to 500% increase in <br />concentration observed during thyroid surges. <br /> <br />We believe that two factors contributed to the error noted in <br />duplicate samples. First, dried samples were re-suspended in ethanol <br />solution. Ethanol solutions are more viscous than aqueous solutions and <br />therefore more difficult to pipette with uniform accuracy. Second, an <br />8.80/0 error was associated with pi petting 25,.11 samples. The mean percent <br />error (:t 95% CI) of duplicate samples (8.2 :t 1.5%) was uniform with the <br />8.8 percent error noted in pipetting the 25j.L1 samples. <br /> <br />The T 4 surges observed in flannel mouth suckers at hatching and <br />swim-up were similiar those seen in a variety of other species, including <br />razorback suckers (Scholz et al. 1982a), kokanee salmon -- Oncorhynchus <br />nerka (Walbaum) -- (Scholz et al. 1982b), pink salmon -- Oncorhynchus <br />gorbuscha (Walbaum) -- (Sullivan et al. 1983), chum salmon -- <br />Oncorhynchus keta (Walbaum) -- (Tagawa and Hirano 1987, 1989), <br />Cyprinidae and Centrarchidae (Tagawa st al. 1990), and Pleuronectidae <br />(Tanaka et al. 1991). Thyroid hormones surges in these species were <br />associated with metamorphic events and imprinting and may serve a <br />similiar function in flannelmouth suckers. <br /> <br />16 <br />