7766

• UCREFRP Catalog Number: 7766
• Author: Scholz, A. T., et al.
• Title: Notes on the Development and Measurement of Egg and Larval Thyroxine Concentration in Flannelmouth Suckers ‎(
• USFW Year: 1992.
• Copyright Material: NO

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addrng--1 ml 1251-T4 to two plain test tubes that did not
contain Ab (uncoated tubes). Tubes were incubated for 1 h @
370 and radioactivity decanted into a radioactive waste
container. The tubes were then blotted and remaining
radioactivity counted. The amount of radioactivity remaining
was considered non-specific binding. The program contained
on the Biogamma counter automatically subtracted the mean
NSB from each standard curve and unkown sample before
calculating percent bound

Ten blank tubes, i.e., tubes that did not contain 1251-T4 or T4
were assayed to ensure that the gamma counter was
functioning properly. Distilled water was added to four Ab
coated tubes and incubated for 1h @ 370C. The tubes were
decanted, blotted and counted like other tubes.

All standard curve, unknown and quality control standards
were assayed in duplicate to control for procedural errors. For
each pair of samples the mean value was calculated and
percent error from the mean determined. The mean percent
error :I: 95% CI for all samples was calculated using Statview
II (Brainpower, Inc.) on an Apple II CX computer. A frequency
distribution was plotted of the percent error.

To determine pipetting accuracy, individuals pipetting the
25~1 unknown/standard curve samples and 1 ml 1251-T4
samples were each required respectively to pipette 10
replicate 25j.1.1 samples or 10 replicate 1 ml sample of 12 SI_
T 4 into uncoated tubes. The amount of radioactivity in each
sample was counted in the gamma counter. The mean (:I: 95%
CI) and coefficient of variation were determined for each set
of samples.

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