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FISH MONITORING <br />CHIC <br />M <br />N N <br />n <br />U) <br />? o <br />0 <br />_.- ....... .- - <br />se <br />OF <br />-- dec <br />10 15 20 <br /># Runs <br />NICK <br />M <br />N N <br />O. <br />? O <br />O <br /> <br />10 15 20 <br /># Runs <br />O <br />co <br />2) <br />N N <br />a <br />V7 <br />? o <br />0 <br />co <br />A) <br />lV N <br />a <br />U) <br />yk O <br />O <br />10 15 20 <br /># Runs <br />271 <br />Figure 5. Selected species accumulation curves generated from 20 electrofishing runs in inflow zones. Reservoirs sampled in supplementary <br />study in 1992 were Chickamauga, Kentucky, Nickajack and Watts Bar. Note asymptote is not achieved before 15 runs, suggesting that <br />samples with only 10 runs are not adequate to characterize fish assemblages present at a site <br />for RFAI scores across months (Figure 3). If the measurement error had been high relative to the observed <br />monthly variability, we would conclude that the RFAI is too sensitive to slight differences in species com- <br />position or abundances within the fish assemblage. On the contrary, the high monthly variability suggests <br />a source of variability other than measurement error. <br />Resampling at the level of the electrofishing run by choosing 10 of 20 available runs provides an estimate of <br />sampling error, or variation in scores resulting from random variability among run locations. The measure- <br />ment error is generally less than the sampling error for the autumn 1992 supplemental data set (Figure 4). <br />The availability of 20 electrofishing runs allowed the construction of species accumulation curves (Figure 5). <br />These curves indicate the rate at which new species are being added to the sample for each electrofishing run. <br />A sampling plan which extends to the asymptote of the curve will provide a more reliable sample than a plan <br />that terminates before the asymptote. In each reservoir, new species were being added to the sample at the <br />15th run. This indicates that the standard sample of 10 runs is insufficient to generate a representative sample <br />of the fish assemblage. We conclude that much of the overall variability in index scores results from inadequate <br />sampling at a site. <br />DISCUSSION <br />A successful RFAI can be used to assess resource condition and even diagnose the factors responsible for <br />degradation. In addition, it can track reservoir condition following management actions. As a monitoring <br />tool, it may alert managers to problem areas that require further, more detailed investigation. To be <br />successful, however, a multimetric index must overcome three challenges: (1) the index must incorporate <br />the relevant biology so that it provides a meaningful measurement of the status or condition of the fish <br />assemblage; (2) the samples on which the index is based must accurately represent critical and relevant <br />attributes of the sampled fish assemblage; and (3) the index scores must be a sensitive integrator of biological <br />responses to stress from human-induced impacts. <br />KENT <br />----___- <br />:.. ............... <br />10 15 20 <br /># Runs <br />WATT <br />