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FISH MONITORING 269 <br />level of the individual fish. Twenty runs were sampled with replacement until a sample of 10 runs was <br />selected. The RFAI scores were calculated for 1000 of these samples and the sampling error was estimated <br />from the distribution of RFAI scores. <br />RESULTS <br />Variation in reservoir rankings, 1989-1992 <br />Although some mainstem reservoirs were consistently ranked among the three sample years, the scores <br />for most reservoirs fluctuated substantially. We limit the discussion to the general statistical properties of the <br />data set rather than specifics of individual sites. Annual rankings of two metrics (PTOL, TLIT) were <br />consistent among years in all three reservoir zones (Table II). Most other metrics ranked reservoirs consis- <br />tently across years in at least one reservoir zone; however, neither FHAI nor TAXA ranked reservoirs <br />consistently in any of the three zones. Ranks of overall reservoir index scores differed among years <br />(Table II). <br />This overall instability in rankings raises questions about those reservoirs and regions that did rank con- <br />sistently among years. On closer examination we find that reservoirs with consistent RFAI scores exhibit <br />considerable variation in metric scores. That is, apparent RFAI stability conceals changes in metrics scores <br />that cancelled each other out, a situation that is rarely encountered in the behaviour of stream IBI scores for <br />fish or insects (J. R. Karr, personal observation). <br />Bootstrap estimation of variance of RFAI scores <br />Four major sources of variability influence RFAI scores: sampling error, measurement error and temporal <br />and spatial variability in resident fish assemblages resulting from natural events or anthropogenic influences. <br />Bootstrap sampling provides an estimate of RFAI's measurement error, or precision. Patterns within and <br />among reservoirs are not strong nor consistent (Table III). The average measurement error for a particular <br />reservoir is not consistently high or low across inflow, transition and forebay; neither are reservoir regions <br />consistently higher or lower across reservoirs. We conclude that measurement error is independent of <br />location and reservoir. <br />1992 supplemental data set <br />The RFAI scores calculated for samples taken during 1992 failed to consistently rank the four reservoirs <br />from September to December (Figure 2). Differences in scores were not associated with any obvious seasonal <br />pattern; the direction of monthly changes in scores differed among reservoirs. The rank orders of the four <br />0 <br />0 <br />0 <br />LO <br />m <br />`o <br />rn <br />0 <br />v <br />Q <br />u_ <br />a: <br />co <br />0 <br />N <br />., <br />. ......................... <br />i <br />?? - ?pnt <br />Ick <br />Sep Oct Nov Dec <br />Figure 2. Reservoir fish assemblage index (RFAI) scores for monthly supplemental collections from Chickamauga, Kentucky, Nicka- <br />jack and Watts Bar reservoirs for September to December 1992. The RFAI scores were calculated with 10 runs closest to the dam <br />(upstream). All samples for a reservoir used the same fish health assessment index (FHAI) score