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61 The C. radiosus population in the Fish Slough refugium appears to be in good condition, as do those in the B.L.M. Spring and Warm Springs refugia. However, numbers in the latter refugia are much smaller than in the main sanctuary, and their populations are much more vulnerable to vandalism. In order that the integrity of the species may be assured, it is necessary that the 200 acres of privately held land be acquired at the earliest possible date. David K. Young, a graduate student at California State University, Fresno is conducting a study of the population dynamics of C. radiosus in B.L.M. Spring. He has completed one season of field work and will continue his field studies in 1978. 1. Tecopa pupfish, Cyprinodon nevadensis calidae. Doug Selby's report follows: An extensive survey of all existing aquatic habitats in the Tecopa, California area was undertaken for California Fish and Game during the summer of 1977. This survey failed to locate any populations of pupfish which could be clearly identified as C. n. calidae. All pupfish examined appeared to be the more common Amargosa pupfish, C. n. amargosae native to the marsh and river below the habitat of C. n.?calidae. It is reasonable to assume that the Tecopa pupfish is extinct. M. Cottonball Marsh pupfish, Cyprinodon milleri. Pete Sanchez reported that the population appears to be in good condition. Heavy rains in the desert all season have flooded portions of the floor of Death Valley and lengthened the normal course of Salt Creek. An attempt will be made by aerial reconnaissance to determine whether or not there is, or has been, any mixing of the two waters, inasmuch as mixing of the two fish populations could have enormous impact on the integrity of the species involved. n. Saratoga Springs pupfish, Cyprinodon nevadensis nevadensis. Pete Sanchez reported that both the habitat and its fish population are in good condition and under no current threat. o. Amargosa pupfish, Cyprinodon nevadensis amargosae. The subspecies exists in good numbers throughout the lower Amargosa River. The only known threat at this time is groundwater development in Nevada. p. Salt Creek pupfish, Cyprinodon salinus. The status of this population remains satisfactory. The 3,000 foot boardwalk observation facility continues to be highly successful in improving interpretive aspects of Salt Creek and in protecting the riparian and aquatic habitats. See item (m) above.