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28 <br />The two species seem to be reproductively active at the same <br />time. In February, March, and April (the only months for which <br />specimens are available), the females of both species carry size- <br />graded series of eggs; they are apparently capable of repeated repro- <br />duction over a long period of time. Both species establish breeding <br />territories in the fine vegetation of the marsh, sometimes within 20 cm <br />of each other, and competition for territories may be intense. <br />C. alvarezi is commonly twice the size of M. aporus and is more aggressive. <br />It has been seen to threaten Megupsilon males which were defending <br />territories. <br />The proportion of Cyprinodon to Megupsilon fluctuates greatly, <br />possibly in response to habitat changes. When the pond was visited <br />in 1961, Megupsilon was apparently rare. In 1968, however, there <br />were three times as many Megupsilon as Cyprinodon. In three different <br />years since then, Cyprinodon has been dominant. Population sizes of <br />the two species were estimated in May, 1978, by counting individuals <br />visible in meter-square quadrats in each habitat type. The average <br />density of Cyprinodon in the marsh, based on 73 quadrats, was 8.4 <br />fish per m2 which indicates about 12,180 Cyprinodon in the whole <br />marsh. The average density of Megupsilon was 1.9 fish per m2 which <br />indicates a population of about 2750 individuals in the marsh. These <br />are probably underestimates since many fish were concealed. The <br />proportion of four Cyprinodon to each Megupsilon may be biased toward <br />Cyprinodon, since Megupsilon sometimes takes cover when the former <br />species is present. Neither species occurred in significant numbers <br />in other parts of the pond (based on 50 quadrats). <br />Since the introduction of bass at E1 Potosi, the two pupfishes <br />have been considered threatened, Megupsilon critically so. In May, <br />1978, the situation had improved, but this may have been a temporary <br />response to an unusually wet spring. Efforts to eliminate bass at E1 <br />Potosi with gill nets have proven unsuccessful. It seems imprudent <br />to use more effective means of bass eradication as the pupfish and <br />other endemic organisms might be adversely affected. Preservation <br />efforts will therefore be based on habitat management with the objective <br />of altering predator-prey interactions in favor of pupfish. Research <br />on life histories, habitat requirements, and ecological segregation <br />is currently under way and will provide the basis for a future <br />management program. <br />Acknowledgements.--Field work was supported in part by grants from <br />the National Science Foundation (DEB77-17315) and the Fauna Preservation <br />Society, London (both to R.R. Miller) and by a Scott Turner Award in <br />Earth Science. Ellie Baker Koon assisted in field work in 1977, and <br />R.R. Miller, Edie Marsh, and Salvador Contreras-Balderas and his students <br />from the Universidad Autonoma de Monterrey participated in 1978. <br />Permission to conduct field work in Mexico was kindly granted by the <br />Direccion General de Regiones Pesqueras.