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TABLE OF CONTENTS <br />Executive Summary ........................................................................................2 <br />lntroduction ................................................................................................... ..4 <br />Hybridization in native fishes ................................................................ ..6 <br />Materials and Methods .................................................................................. ..8 <br />Sampling .............................................................................................. ..8 <br />DNA extraction ..................................................................................... ..8 <br />Intron Development .............................................................................. ..9 <br />SnapShot .............................................................................................. 10 <br />Data Analysis ....................................................................................... 11 <br />Results .......................................................................................................... 11 <br />Samples ............................................................................................... 11 <br />Intron variation ...................................................................................... 12 <br />Species composition based on phenotype ........................................... 13 <br />Species composition based on genotype ............................................. 13 <br />Size distribution .................................................................................... 15 <br />Discussion .................................................................................................... 15 <br />Hybrid detection ...................................................................................15 <br />Use of introns .......................................................................................16 <br />Screening for hybrids ...........................................................................17 <br />SNP screening for hybrids ....................................................................18 <br />Yampa River sucker hybrid zone ..................:...........:...........................18 <br />Badccrossing and introgression ............................................................19 <br />Conclusions and management implications .........................................20 <br />Acknowledgments .........................................................................................21 <br />Literature Cited .............................................................................................22 <br />Tables ........................................................................................................... 30 <br />Figures .......................................................................................................... 37 <br />Glossary ........................................................................................................48 <br />1 <br />