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' lithium, uranium, selenate, and boron) suggest a high toxicity to the swim-up fry <br />of hatchery-reared razorback sucker, Colorado squawfish, and bonytail (Appendix <br />C). Toxicity tests were conducted in water that simulated conditions in the <br />middle Green River near Jensen, Utah. This area had elevated concentrations of <br />some trace elements in water, bottom sediments, and biological tissue (fish, <br />aquatic invertebrates, and plants). <br />The next logical step would be to conduct in situ bioassays using the most <br />vulnerable life stages of the species of concern. It is beneficial to have a <br />site to conduct in situ studies to access bioaccumulation in and toxicity of <br />selected elements to target endangered fishes. This may provide some answers to <br />determine if trace elements are limiting the survival of this species. <br />Razorback sucker of different sizes ranging from post-larvae to fingerlings <br />could be stocked into Site 1 at the Escalante SWA. Larvae of two other native <br />suckers (flannelmouth and bluehead) could also be stocked in this site for in <br />' situ bioassays. Fish used in these studies would be progeny from upper Colorado <br />River stock. A small sample of fish could be retrieved and sacrificed for <br />contaminant analysis at various intervals until the fish reach maturity. <br />Attempts would be made to monitor chronic effects of trace elements. A <br />laboratory would serve as the control site. In the laboratory, the <br />concentrations and combinations of the various trace elements could be <br />' controlled. The warmwater, productive area should grow fish rather quickly. <br />Fish would probably reach maturity in about 2-1/2 years following stocking. <br />After reaching maturity, gametes and F, progeny from these fish reared in this <br />site could be analyzed for selected trace elements. The bioaccumulation and <br />' concentration of trace elements thought to be harmful could be analyzed and <br />compared among life stages and among the three native sucker species for both the <br />control and "on-site" studies. The on-site or in situ studies would allow <br />' assessment of dietary uptake of potential trace elements and their impacts to the <br />three native sucker species. In the laboratory studies, growth and reproductive <br />"vigor" of these three native sucker species subjected to the various trace <br />' elements (singly or in combination) and concentrations could be compared to the <br />control group (subjected to 'background concentrations' of trace elements). <br />Contaminant biologists would be responsible for designing and conducting these <br />in situ studies. <br />' This information is critical in determining if selenium or other trace <br />elements contribute to mortality of razorback sucker in the Gunnison River. If <br /> toxicity concentrations of these trace elements are shown to be deleterious to <br />' certain life stages, attempts will be made to remedy the accumulation of trace <br /> elements that are harmful to the endangered fishes. This information will be <br />' useful a <br />be used s screening criteria for selection of a site <br />for recover or river reach that could <br /> y. <br />Information on survival of razorback sucker stocked at various sizes from <br />' larvae to fingerling and in the presence or absence of predatory non-native fish <br />that occur in the river could be evaluated at this site. The three compartments <br />would allow replicate experiments to be conducted with controls. Also, fish <br />' stocked as larvae could be reared and tagged for restoration stocking of the <br />Gunnison River. Tagging and marking endangered fishes will follow Program <br />protocol. Food production and invertebrate indices would be monitored seasonally <br />' 21 <br />