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ISMP backwater sampling evaluation <br />surface area, had minimum depth of 10 cm if turbid or 30 cm if clear, and were not flowing, as <br />per ISMP selection standards. Each backwater was blocknetted at the mouth and the fish <br />community sampled with two non-overlapping ISMP seine hauls (Fig. 3). The hauls were <br />typically made near the mouth and nearer the apex and were usually transverse to the long axis of <br />the backwater. Fish in ISMP samples were identified and enumerated in the field or preserved in <br />10 % formalin for processing in the laboratory. Area seined was measured. <br />The backwater fish community was then sampled again using a more intensive 3-pass <br />seine depletion or capture-recapture approach. In seine depletion backwaters, seine hauls (4.6 m <br />long seine, 3 mm mesh) were made in sufficient number to sample nearly the entire area of the <br />backwater on each of three passes; the same number of seine hauls (effort) was made on each <br />sampling pass. Fish captured on each pass were placed in separate live baskets and were <br />enumerated or preserved for laboratory analysis when all sampling passes were completed. <br />Before preservation all samples were scanned for native fishes which were enumerated and <br />released. Preserved samples were identified and enumerated at the Larval Fish Laboratory, <br />Colorado State University, Fort Collins. <br />Capture-recapture sampling involved capturing fish on one or two consecutive days <br />(passes) with some combination of seining or fyke nets and was followed with a final pass which <br />employed fyke nets, electrofishing, or both. Fyke nets were used in 1997 but were found <br />relatively inefficient because they captured few fish, were size and species selective, and hence, <br />were not used in 1998 sampling. Green sunfish and largemouth bass sampled on all but the final <br />pass were marked with a unique fin clip. In backwaters where capture-recapture sampling was <br />employed, other fish species were incidentally captured and enumerated and were included in <br />-4-